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Balance between DNA-binding affinity and specificity enables selective recognition of longer target sequences in vivo
https://repo.qst.go.jp/records/76503
https://repo.qst.go.jp/records/765035284b26c-b7f8-4d7f-bfd0-7880ef46b281
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-04-25 | |||||
| タイトル | ||||||
| タイトル | Balance between DNA-binding affinity and specificity enables selective recognition of longer target sequences in vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tomoko, Sunami,
× Tomoko, Sunami,× Hidetoshi, Kono,× Sunami, Tomoko× Kono, Hidetoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although genome-editing enzymes such as TALEN and CRISPR/Cas9 are being widely used, they have an essential limitation in that their relatively high molecular weight makes them unsuitable for capsulation. To develop a novel genome-editing enzyme with a smaller molecular weight, we focused on the engrailed homeodomain (EHD). We designed and constructed proteins composed of two EHDs connected by a linker to increase sequence specificity. In bacterial one-hybrid assays and EMSA analyses, the created proteins exhibited good affinity for DNA sequences consisting of two tandemly aligned EHD target sequences. However, they also bound to individual EHD targets. To avoid binding to single target sites, we introduced amino acid mutations to reduce the protein-DNA affinity of each EHD monomer and successfully created a small protein with high specificity for tandem EHD target sequences. | |||||
| 書誌情報 |
Protein Science 巻 28, 号 9, p. 1630-1639, 発行日 2019-08 |
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| 出版者 | ||||||
| 出版者 | The Protain Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0961-8368 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/pro.3677 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.3677 | |||||
