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The Role of XRCC4 in Human Colon Cancer Stem Cells and Radiosensitivity
https://repo.qst.go.jp/records/76099
https://repo.qst.go.jp/records/7609963ac2a56-6a07-4ce2-8ef5-5ad8e3b0da89
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-06-25 | |||||
| タイトル | ||||||
| タイトル | The Role of XRCC4 in Human Colon Cancer Stem Cells and Radiosensitivity | |||||
| タイトル | ||||||
| タイトル | 鈴木 雅雄 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Vares, Guillaume× Hayashi, Mitsuhiro× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Vares, Guillaume |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: The X-ray cross-complementing group 4 (XRCC4) is a major gene in the nonhomologous DNA end-joining (NHEJ) DNA repair pathway. Here we attempt to investigate the relationship between XRCC4 and cancer stem cell (CSC) properties and the involvement of XRCC4 in the radiosensitivity of human colon CSCs to carbon-ion beams or X-ray irradiation. Methods: Putative CSCs sorted from HCT116-wild type (WT) and XRCC4-KO cells were treated with or without carbon-ion beams or X-ray irradiation, then the colony and spheroid formation assay, fluorescence activated cell sorting (FACS) analysis and H2AX foci assay were performed. Results: We found that the percentage of CSC markers CD44+ and ESA+ cells had significantly increased in XRCC4 KO cells compared to HCT116-WT cells. The number of colony and spheroid formed from CSCs was significantly higher compared to those from non-CSCs in XRCC4-KO cells, but markedly decreased compared with those from HCT116-WT cells. The doses at D10 levels of CSCs sorted from XRCC4-KO and HCT116-WT cells were 1.4 and 4.2 Gy for X-ray and 1.0 and 1.9 Gy for carbon ion beam, respectively. A much larger number and large-sized gamma H2AX foci were observed in CSCs sorted from XRCC4 KO cells compared to those from HCT116-WT cells, after 24 h of irradiation with carbon ion beam compared with X-ray. Conclusion: Taken together, lack of XRCC4 significantly altered CSC properties, and radiosensitized CSCs to both carbon-ion beams and X-rays, suggesting that XRCC4 may plays a pivotal role in modulating radiosensitivity of CSCs. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 45th Annual Meeting of Korean Cancer Association and the 5th International Cancer Conference | |||||
| 発表年月日 | ||||||
| 日付 | 2019-06-21 | |||||
| 日付タイプ | Issued | |||||
