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  1. 原著論文

Observation of Protein and Lipid Membrane Structures in a Model Mimicking the Molecular Crowding Environment of Cells Using Neutron Scattering and Cell Debris

https://repo.qst.go.jp/records/75692
https://repo.qst.go.jp/records/75692
6380286d-fcfc-4608-800c-870ae9b76bfb
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-03-29
タイトル
タイトル Observation of Protein and Lipid Membrane Structures in a Model Mimicking the Molecular Crowding Environment of Cells Using Neutron Scattering and Cell Debris
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Hirai, Mitsuhiro

× Hirai, Mitsuhiro

WEKO 748583

Hirai, Mitsuhiro

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Ajito, Satoshi

× Ajito, Satoshi

WEKO 748584

Ajito, Satoshi

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Arai, Shigeki

× Arai, Shigeki

WEKO 748585

Arai, Shigeki

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Adachi, Motoyasu

× Adachi, Motoyasu

WEKO 748586

Adachi, Motoyasu

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Shimizu, Rumi

× Shimizu, Rumi

WEKO 748587

Shimizu, Rumi

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Wakamatsu, Kaori

× Wakamatsu, Kaori

WEKO 748588

Wakamatsu, Kaori

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Shin-ichi, Takata

× Shin-ichi, Takata

WEKO 748589

Shin-ichi, Takata

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Iwase, Hiroki

× Iwase, Hiroki

WEKO 748590

Iwase, Hiroki

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Arai, Shigeki

× Arai, Shigeki

WEKO 748591

en Arai, Shigeki

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Adachi, Motoyasu

× Adachi, Motoyasu

WEKO 748592

en Adachi, Motoyasu

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Shimizu, Rumi

× Shimizu, Rumi

WEKO 748593

en Shimizu, Rumi

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抄録
内容記述タイプ Abstract
内容記述 The interior of living cells is a molecular-crowding environment, where large quantities of various molecules coexist. Investigations into the nature of this environment are essential for an understanding of both the elaborate biological reactions and maintenance of homeostasis occurring therein. The equilibrium states of biological macromolecular systems are affected by molecular crowding environments unmatched by in vitro diluted environments; knowledge about crowding effects is still insufficient due to a lack of relevant experimental studies. Recent developments in the techniques of in-cell NMR and large-scale molecular dynamics simulation are providing new insights into the structure and dynamics of biological molecules inside cells. This study focused on a new experimental technique to directly observe the structure of a specific protein or membrane in condensed crowder solutions using neutron scattering. Deuterated whole-cell debris was used to reproduce an environment that more closely mimics the interior of living cells than models used previously. By the reduction of the background-scattering from large amounts of cell debris, we successfully extracted structure information for both a small globular protein and a small unilamellar vesicle (SUV) from the concentrated cell-debris solution up to a weight ratio of 1/60 for protein/crowder and 1/40 for SUV/crowder.
書誌情報 The Journal of Physical Chemistry B

巻 123, 号 15, p. 3189-3198, 発行日 2019-03
出版者
出版者 American Chemical Society
ISSN
収録物識別子タイプ ISSN
収録物識別子 1520-6106
PubMed番号
識別子タイプ PMID
関連識別子 30905155
DOI
識別子タイプ DOI
関連識別子 10.1021/acs.jpcb.8b12546
関連サイト
識別子タイプ URI
関連識別子 https://pubs.acs.org/doi/10.1021/acs.jpcb.8b12546
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