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Observation of Protein and Lipid Membrane Structures in a Model Mimicking the Molecular Crowding Environment of Cells Using Neutron Scattering and Cell Debris
https://repo.qst.go.jp/records/75692
https://repo.qst.go.jp/records/756926380286d-fcfc-4608-800c-870ae9b76bfb
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-03-29 | |||||
| タイトル | ||||||
| タイトル | Observation of Protein and Lipid Membrane Structures in a Model Mimicking the Molecular Crowding Environment of Cells Using Neutron Scattering and Cell Debris | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirai, Mitsuhiro
× Hirai, Mitsuhiro× Ajito, Satoshi× Arai, Shigeki× Adachi, Motoyasu× Shimizu, Rumi× Wakamatsu, Kaori× Shin-ichi, Takata× Iwase, Hiroki× Arai, Shigeki× Adachi, Motoyasu× Shimizu, Rumi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The interior of living cells is a molecular-crowding environment, where large quantities of various molecules coexist. Investigations into the nature of this environment are essential for an understanding of both the elaborate biological reactions and maintenance of homeostasis occurring therein. The equilibrium states of biological macromolecular systems are affected by molecular crowding environments unmatched by in vitro diluted environments; knowledge about crowding effects is still insufficient due to a lack of relevant experimental studies. Recent developments in the techniques of in-cell NMR and large-scale molecular dynamics simulation are providing new insights into the structure and dynamics of biological molecules inside cells. This study focused on a new experimental technique to directly observe the structure of a specific protein or membrane in condensed crowder solutions using neutron scattering. Deuterated whole-cell debris was used to reproduce an environment that more closely mimics the interior of living cells than models used previously. By the reduction of the background-scattering from large amounts of cell debris, we successfully extracted structure information for both a small globular protein and a small unilamellar vesicle (SUV) from the concentrated cell-debris solution up to a weight ratio of 1/60 for protein/crowder and 1/40 for SUV/crowder. | |||||
| 書誌情報 |
The Journal of Physical Chemistry B 巻 123, 号 15, p. 3189-3198, 発行日 2019-03 |
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| 出版者 | ||||||
| 出版者 | American Chemical Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1520-6106 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 30905155 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/acs.jpcb.8b12546 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://pubs.acs.org/doi/10.1021/acs.jpcb.8b12546 | |||||
