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Free energy profiles for unwrapping the outer superhelical turn of nucleosomal DNA under torsional stress
https://repo.qst.go.jp/records/75566
https://repo.qst.go.jp/records/75566b1d27fee-ec8d-48d6-b5cd-81022db51aff
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-04-09 | |||||
| タイトル | ||||||
| タイトル | Free energy profiles for unwrapping the outer superhelical turn of nucleosomal DNA under torsional stress | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishida, Hisashi
× Ishida, Hisashi× Kono, Hidetoshi× Ishida, Hisashi× Kono, Hidetoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Torsional stress gives a significant impact on the structure and dynamics of chromatin. For example, the torsion stress generates supercoiling in chromatin, where negatively supercoiled domains form a decompacted large-scale chromatin structure and are highly transcribed. RNA polymerase imposes torsional stress on DNA in chromatin and unwraps the DNA from the nucleosome to access the genetic information encoded in the DNA. It has also been shown that mechanical torsional stress influences the nucleosome structure and stability. To understand how the torsional stress affects the nucleosome stability, we carried out all atomic molecular dynamics simulations to examine the unwraping of the outer superhelical turn of the nucleosomal DNA from the histone octamer under the torsional stress imposed on the edges of the DNA. First, using an adaptively biased potential method, a series of conformations of the unwrapping DNA from the histone core was generated without torsional stress. Second, the free energies required to unwrap the DNA under positive and negative torsional stresses were estimated using umbrella sampling simulations. It was found that the positive torsion facilitated the unwrapping of the overwound DNA, while the negative torsion facilitated the wrapping of the underwound DNA around the histone core. The difference in these free-energies was observed after the interaction between the DNA and H3 histone was disconnected. The conformational entropy of the unwrapped DNA increased under the negative stress, indicating that the underwound DNA becomes more flexible. The flexibility of the underwound DNA may facilitate the movement of the unwrapped DNA back to the histone core in the stable state. We concluded that the torsion stress has a significant impact on the free energy of the unwrapping DNA and its dynamics, which would contribute to the conformational change of chromatin. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Multiscale Modeling of Chromatin: Bridging Experiment with Theory - Les Houches 2019 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-04-03 | |||||
| 日付タイプ | Issued | |||||
