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Mutagenesis of the Oil-producing Algae by Ion Beam Irradiation
https://repo.qst.go.jp/records/74761
https://repo.qst.go.jp/records/74761334504b1-3838-4ad3-bccc-31695b752d2a
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-03-15 | |||||
| タイトル | ||||||
| タイトル | Mutagenesis of the Oil-producing Algae by Ion Beam Irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hiroya, Araie,
× Hiroya, Araie,× 長谷, 純宏× Yasushi, Iwata,× 大野, 豊× Iwane, Suzuki,× Hase, Yoshihiro× Ono, Yutaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For biofuel production using microalgae, it is necessary to mutate the algae to improve the productivity of biomass and oil contents. In this study, we focused on very-long-alkyl ketones so-called alkenone that are thought to be good candidates for biofuels. Previously, we reported dose-dependency of the colony formation after 0-320 Gy of carbon ion irradiation. We screened mutants that showed high alkenone productivity from the irradiated cells. In this study, we irradiated carbon ion beams to the high alkenone-producing mutants to investigate the effects of the second-round mutagenesis on the mutant. The cells cultured for 14 d in a 96-well plate were stained with Nile Red and the amount of lipids was evaluated by a microplate reader. The results suggested that it is possible to further improve the productivity of high alkenone-producing strain by the second-round mutagenesis. In the precious study, 30 Gy was suggested to be a suitable dose to enhance oil productivity, however, it seemed better to use a lower dose for the second-round irradiation. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2017 p. 89, 発行日 2019-03 |
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