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Effective Eradication of Cancer Stem-Like Cells by Carbon Ion Beams Combined With DNA-Damaging Drugs
https://repo.qst.go.jp/records/73252
https://repo.qst.go.jp/records/73252bf54b7ce-0d63-4756-a927-83773711f400
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-12-17 | |||||
| タイトル | ||||||
| タイトル | Effective Eradication of Cancer Stem-Like Cells by Carbon Ion Beams Combined With DNA-Damaging Drugs | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
崔, 星
× 崔, 星× 崔 星 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although a relatively small proportion of all breast cancer (BC), triple negative (TN) BC is responsible for a relatively large proportion of BC deaths because of its worse clinical outcome. In this study, we try to investigate whether a carbon ion beam alone or in combination with cisplatin has a beneficial effect compared to X-rays, we target triple negative (TN) breast cancer stem-like cells (CSCs). We found that the colony, spheroid formation, and tumorigenicity assays confirmed that CD44+/CD24- and CD44+/ESA+ cells have exactly the same CSC properties as CD44-/ CD24- and CD44-/ESA- cells. Carbon ion beam combined with cisplatin significantly suppressed colony and spheroid formation compared to X-rays combined with cisplatin or carbon ion beam alone. RT-PCR Array analysis showed that carbon ion beam combined with cisplatin significantly induced apoptosis-related Cytochrome c, almost completely eliminated expression of the CSC markers CD44 and ESA, and significantly inhibited angiogenesis, and metastasis-related HIF1α and CD26 compared to carbon ion beam alone, X-ray alone, or X-ray combined with cisplatin. The immunofluorescence assay showed that not only the number but also the size of γH2AX foci in CSCs were larger 24 h after carbon ion beam combined with cisplatin compared to those of X-ray combined with cisplatin and carbon ion beam alone. In conclusion, carbon ion beam combined with cisplatin has superior potential to kill TN breast CSCs with irreparable severe DNA damage and enhanced apoptosis. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SPHIC-NIRS Joint Symposium | |||||
| 発表年月日 | ||||||
| 日付 | 2015-12-10 | |||||
| 日付タイプ | Issued | |||||
