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Disruption of Aspm causes microcephaly with abnormal neuronal differentiation
https://repo.qst.go.jp/records/73228
https://repo.qst.go.jp/records/732281d7c0355-eca2-4e34-8d71-f38be23d7d51
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-02-23 | |||||
| タイトル | ||||||
| タイトル | Disruption of Aspm causes microcephaly with abnormal neuronal differentiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujimori, Akira
× Fujimori, Akira× al., et× 藤森 亮 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A number of ASPM mutations have been detected in primary microcephaly patients. In order to evaluate the function of ASPM in brain development, we generated model animals of human autosomal recessive primary microcephaly-5 (MCPH5). In the Aspm knock-out mice, the exon 2–3 of the Aspm gene was encompassed by a pair of loxP signals so that cre-recombinase activity switched the allele from wild-type to null zygotes as frequently, as expected from the Mendelian inheritance. We precisely analyzed the brains of adults and fetuses using immunohistochemistry and morphometry. The adult brains of the Aspm-/- mice were smaller, especially in the cerebrum. In the barrel field of the somatosensory cortex, layer I was significantly thicker, whereas layer VI was significantly thinner in Aspm-/- mice, compared with Aspm+/+ mice. The total number of cells and the thickness of the cortical plate at embryonic day 16.5 was significantly decreased in Aspm-/- mice, compared with Aspm+/+ mice. Furthermore, the expression of transcription factors, such as Tbr1 and Satb2, was significantly increased in the subplate of the Aspm-/- mice. The results suggested that Aspm is essential to the proliferation and differentiation of neural stem/pro-genitor cells. The Aspm gene loss model provided a novel pathogenetic insight into acquired microcephaly, which can be caused by in utero exposure to both known and unknown teratogens. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第5回国際放射線神経生物学会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2015-02-21 | |||||
| 日付タイプ | Issued | |||||
