WEKO3
アイテム
DETERMINATION OF PROTEIN STRUCTURE IN MIMIC-CELL MOLECULAR CROWDING
https://repo.qst.go.jp/records/73145
https://repo.qst.go.jp/records/73145b2eec913-7168-445f-bd30-a37f4fb13810
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-01-15 | |||||
| タイトル | ||||||
| タイトル | DETERMINATION OF PROTEIN STRUCTURE IN MIMIC-CELL MOLECULAR CROWDING | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirai, M.
× Hirai, M.× Ajito, S.× 新井, 栄揮× Takata, S.× Iwase, H.× 新井 栄揮 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The interior of living cells is in so-called molecular-crowding environments where large amounts of various molecules coexist in about 30-40 % v/v. The stability of proteins in aqueous solutions is well known to vary by the addition of salts and neutral substances, such as sugars and polyols. Thus, crowding environment has been considered to affect the equilibrium states of proteins, whereas, interpretations of molecular-crowding effect remain controversial because most of the experimental studies of protein structure and stability such as unfolding-folding have been done under dilute-solution conditions. Recently, we have obtained direct evidence that sugar and polyol effect to preserve hydration-shell of protein by preferential exclusion of those molecules from a protein surface [1, 2]. Based on our previous results using neutron and X-ray scattering, we have executed further experiments to clarify structural characteristics of proteins in a mimic-cell environment that was realized by using the contents of deuterated whole cells. Deuterated whole cells were homogenized by sonication and were used as mimic-cell crowders. Neutron scattering measurements were carried out by using the BL15 TAIKAN spectrometer at the pulsed-neutron source of the Materials and Life Science Experimental Facility (MLF) at the Japan Proton Accelerator Research Complex (J-PARC, Tokai, Japan). The neutron wavelength was 0.5 - 6.0 Å. In the neutron scattering experiments, we have employed the inverse-contrast variation method in neutron scattering. This method using deuterated materials is known to avoid or minimize the artificial effect on the scattering curves caused by the presence of co-solute molecules. We have also done synchrotron radiation wide-angle X-ray scattering (SRWAXS) measurements by using the BL-10C spectrometer at the High Energy Accelerator Research Organization (KEK, Tsukuba, Japan). We have succeeded to observe only a protein structure by mostly diminishing the effect of mimic-cell environment. \nReferences [1] M. Hirai et al., Physica B, (2018) doi.org/10.1016/j.physb.2018.02.020 [2] S. Ajito, M. Hirai et al., Physica B, doi.org/10.1016/j.physb.2018.03.040. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SAS2018 | |||||
| 発表年月日 | ||||||
| 日付 | 2018-10-08 | |||||
| 日付タイプ | Issued | |||||
