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Neutron structure analysis of NADH cytochrome b5 reductase
https://repo.qst.go.jp/records/72980
https://repo.qst.go.jp/records/72980ee8aa47f-004d-4460-ac27-3f7419f165eb
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-10-18 | |||||
| タイトル | ||||||
| タイトル | Neutron structure analysis of NADH cytochrome b5 reductase | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
平野, 優
× 平野, 優× 栗原, 和男× Ostermann, Andreas× 日下, 勝弘× 木村, 成伸× 三木, 邦夫× 玉田, 太郎× 平野 優× 栗原 和男× 玉田 太郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Redox proteins functions in many biological processes, such as metabolism, photosynthesis, respiration. The chemical reactions of redox proteins proceed with small structural changes involved in hydrogen atoms and/or valence electrons. Thus, high-resolution structure analysis revealing these small structural changes is necessary for understanding molecular mechanisms of redox reactions. NADH cytochrome b5 reductase (b5R) catalyzes the electron transfer from two electron carriers of NADH to one electron carrier of cytochrome b5 (b5). High-resolution X-ray structure analyses have been reported for b5R and b5 from porcine liver. Recently, we have performed high-resolution neutron crystal structure analyses of b5R. We succeeded in data collection of b5R (oxidized form) at high resolutions, 1.40 Å (at iBIX in J-PARC) and 1.45 Å (at BIODIFF in FRM-II), under cryogenic conditions. The logarithmic plot of average diffraction intensities against resolution shows gradual decrease from low to high resolutions in the BIODIFF data, in contrast the plot shows a flat line at the high resolution side in the iBIX data. We have performed the correction of diffraction intensities for the iBIX data using the BIODIFF data. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | German Conference for Research with Synchrotron Radiation, Neutron and Ion Beams at Large Facilities 2018 | |||||
| 発表年月日 | ||||||
| 日付 | 2018-09-17 | |||||
| 日付タイプ | Issued | |||||
