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Expression of translesion DNA polymerase genes from Deinococcus grandis in Escherichia coli
https://repo.qst.go.jp/records/72957
https://repo.qst.go.jp/records/729576e21dd2f-499e-4fab-96a6-a1b3b1ddd171
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-09-26 | |||||
| タイトル | ||||||
| タイトル | Expression of translesion DNA polymerase genes from Deinococcus grandis in Escherichia coli | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sanzen, Toshihiko
× Sanzen, Toshihiko× 佐藤, 勝也× Narumi, Issay× 佐藤 勝也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | While Deinococcus radiodurans, the type species of the genus Deinococcus, does not possess any genes involved in translesion DNA synthesis, the draft genome sequencing of Deinococcus grandis KS0485 revealed that this bacterium possesses two lexA-imuB-dnaE2 gene clusters, which will be involved in translesion DNA synthesis. In this study, we expressed deinococcal dnaE2 and imuB genes in Escherichia coli to further delineate the function of these genes. The dnaE2 and imuB genes were cloned in pET-based vector and p15A-base vector, respectively, and introduced in E. coli BL21(DE3) cells. Mutant frequency was measured by colony formation assay based on rifampicin resistance. As a result, when both the dnaE2 and imuB genes were expressed in E. coli under IPTG induction, the mutant frequency was increased, confirming that the functionality of dnaE2 and imuB as mutagenesis genes. However, when dnaE2 or imuB gene was separately expressed in E. coli, no increase in mutant frequency was observed. These results suggest that both gene products are necessary to operate mutagenesis. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第12回極限環境生物国際会議(Extremophiles2018)出席 | |||||
| 発表年月日 | ||||||
| 日付 | 2018-09-19 | |||||
| 日付タイプ | Issued | |||||
