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Structural and functional analysis of carbohydrate hydrolases from Eisenia fetida

https://repo.qst.go.jp/records/72916
https://repo.qst.go.jp/records/72916
720d6c67-a740-4602-92ab-7d114ca67063
Item type 会議発表用資料 / Presentation(1)
公開日 2018-09-04
タイトル
タイトル Structural and functional analysis of carbohydrate hydrolases from Eisenia fetida
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 中, 裕規

× 中, 裕規

WEKO 718501

中, 裕規

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平野, 優

× 平野, 優

WEKO 718502

平野, 優

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中澤, 昌美

× 中澤, 昌美

WEKO 718503

中澤, 昌美

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阪本, 龍司

× 阪本, 龍司

WEKO 718504

阪本, 龍司

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玉田, 太郎

× 玉田, 太郎

WEKO 718505

玉田, 太郎

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上田, 光宏

× 上田, 光宏

WEKO 718506

上田, 光宏

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平野 優

× 平野 優

WEKO 718507

en 平野 優

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玉田 太郎

× 玉田 太郎

WEKO 718508

en 玉田 太郎

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抄録
内容記述タイプ Abstract
内容記述 Earthworms belong to the phylum Annelida and are known to hydrolyze carbohydrates, which contributes to their ability to digest leaf litter, roots, yeast, brown algae, and fungi in soil. We previously reported that some carbohydrate hydrolases of Eisenia fetida showed the high activities in cold conditions. In addition, the endo-1,4-beta-glucanase and raw starch-digesting amylases, chitinase and beta-1,4-mannanase gene from E. fetida were cloned, expressed in Pichia pastoris (1, 2, 3, 4). The crystal structures of beta-1,4-glucanase and raw starch-digesting amylase (EF-AmyI) and beta-1,4-mannanase have been already analyzed (4, 5, 6). The negatively charged amino acids (Asp and Glu) of beta-1,4-glucanase (EF-EG2) occupied over two-thirds of accessible surface area from the total charged amino acids. It seems that the highly negatively charged surface of EF-EG2 contributes to the cold adaptation. We have applied site-directed mutagenesis (mutation of salt bridges) to improve the catalytic efficiency of beta-1,4-mannnanase at cold condition. It found that salt bridge formed by R318 played an important role for the increase of catalytic activity at low temperature condition.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 14th International Chitin and Chitosan Conference
発表年月日
日付 2018-08-29
日付タイプ Issued
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