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Structural and functional analysis of carbohydrate hydrolases from Eisenia fetida
https://repo.qst.go.jp/records/72916
https://repo.qst.go.jp/records/72916720d6c67-a740-4602-92ab-7d114ca67063
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-09-04 | |||||
| タイトル | ||||||
| タイトル | Structural and functional analysis of carbohydrate hydrolases from Eisenia fetida | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
中, 裕規
× 中, 裕規× 平野, 優× 中澤, 昌美× 阪本, 龍司× 玉田, 太郎× 上田, 光宏× 平野 優× 玉田 太郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Earthworms belong to the phylum Annelida and are known to hydrolyze carbohydrates, which contributes to their ability to digest leaf litter, roots, yeast, brown algae, and fungi in soil. We previously reported that some carbohydrate hydrolases of Eisenia fetida showed the high activities in cold conditions. In addition, the endo-1,4-beta-glucanase and raw starch-digesting amylases, chitinase and beta-1,4-mannanase gene from E. fetida were cloned, expressed in Pichia pastoris (1, 2, 3, 4). The crystal structures of beta-1,4-glucanase and raw starch-digesting amylase (EF-AmyI) and beta-1,4-mannanase have been already analyzed (4, 5, 6). The negatively charged amino acids (Asp and Glu) of beta-1,4-glucanase (EF-EG2) occupied over two-thirds of accessible surface area from the total charged amino acids. It seems that the highly negatively charged surface of EF-EG2 contributes to the cold adaptation. We have applied site-directed mutagenesis (mutation of salt bridges) to improve the catalytic efficiency of beta-1,4-mannnanase at cold condition. It found that salt bridge formed by R318 played an important role for the increase of catalytic activity at low temperature condition. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Chitin and Chitosan Conference | |||||
| 発表年月日 | ||||||
| 日付 | 2018-08-29 | |||||
| 日付タイプ | Issued | |||||
