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The Importance of DNA Double Strand Break Repair Defects to Cell Radiosensitivity in Helium and Carbon Ion Beams
https://repo.qst.go.jp/records/72876
https://repo.qst.go.jp/records/728763a978a70-4d35-4b79-a786-f7ffcfb3156a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-07-29 | |||||
| タイトル | ||||||
| タイトル | The Importance of DNA Double Strand Break Repair Defects to Cell Radiosensitivity in Helium and Carbon Ion Beams | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Flint, David
× Flint, David× McFadden, Conor× Bright, Scott× Yoon, David× Kodaira, Satoshi× Konishi, Teruaki× sawakuchi, Gabriel× 小平 聡× 小西 輝昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To assess the importance of DNA double strand break (DSB) repair defects on cell radiosensitivity in helium and carbon ion beams of varying linear energy transfer (LET). \nMethods: We selected a number of cancer cell lines based upon their deficiency and proficiency statuses in the non‐homologous end joining (NHEJ) and homologous recombination (HR) DSB repair pathways: M059K and M059J glioblastoma cells (extracted from the same tumor, but M059J is deficient in NHEJ), H1299 non‐small cell lung cancer cells (upregulated HR), and as reference cell lines, H460 large cell lung cancer and BxPC3 pancreatic adenocarcinoma cells; and performed clonogenic survival assays in helium and carbon ion beams of varying LETs. The cells were exposed to un‐modulated ion beams with nominal energies of 150 MeV/u (helium) and 290 MeV/u (carbon), using energy degraders to achieve LETs ranging from 2.2 to 60 keV/μm. We exposed the cells to clinical 6 MV x‐rays at a depth of 10 cm in water for reference. Clonogenic survival was then used to determine the relative biological effectiveness (RBE) for each cell line and LET at 10% survival fraction. \nResults: We observed minimal increases in RBE with LET for the NHEJ deficient cells (only 1.34 ± 0.05 for M059J cells at 60 keV/μm), while observing large increases in RBE with LET for the reference cell lines (2.2 ± 0.2 for M059K, 1.78 ± 0.05 for H460 and 3.1 ± 0.6 for BxPC3 at 60 keV/μm). However, the NHEJ deficient cells were the most radiosensitive to all LETs. The cells with upregulated HR, H1299, showed a modest increase in RBE with LET (1.59 ± 0.11 at 60 keV/μm), and were the most radioresistant to all LETs. \nConclusion: Our results indicate that proficiency status in HR and NHEJ may influence how cell radiosensitivity varies with light ion LET. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | THE 2018 AAPM ANNUAL MEETING, 60th Annual Meeting and Exhibition | |||||
| 発表年月日 | ||||||
| 日付 | 2018-08-01 | |||||
| 日付タイプ | Issued | |||||
