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アイテム
Preparation of Selenium-Deficient Mouse Model and its Glutathione Peroxidase Activity
https://repo.qst.go.jp/records/72815
https://repo.qst.go.jp/records/72815821e93e1-4556-4a6b-930f-2d8f81e170dc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-20 | |||||
| タイトル | ||||||
| タイトル | Preparation of Selenium-Deficient Mouse Model and its Glutathione Peroxidase Activity | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ueno, Megumi
× Ueno, Megumi× Nyui, Minako× Emiko, Sekine-Suzuki× Shimokawa, Takashi× Nakanishi, Ikuo× Ken-ichiro, Matsumoto× Ueno, Megumi× Nyui, Minako× Sekine, Emiko× Shimokawa, Takashi× Nakanishi, Ikuo× Matsumoto, Kenichiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Relatively young (8-week-old) selenium deficient (SeD) mouse model was prepared using SeD diet. Selenium (Se), which is an essential trace element, has important roles in the biological redox regulation system as the catalytic center of glutathione peroxidases (GSH-Px). Mice were fed on torula yeast based SeD diet (F2SeDD, Oriental Yeast Co., Ltd., Tokyo) and ultra-pure water (mili-Q water). Several different timings for starting SeD diet were demonstrated and weekly time course of liver GSH-Px activities after starting SeD diet were monitored. Postweaning (4-week-old or older) start of SeD diet gives relatively older (> 8-week-old) SeD mice. The same procedure which was reported for a Se-deficient rat model (Matsumoto et al., Biol. Pharm. Bull. 23, 641-644, 2000.) could not prepared Se-deficient mouse model due to neonatal death. Starting SeD diet to the mother mouse from 2 weeks after giving birth could give 8-week-old SeD mouse model (SeD-trial-2w). Tissue GSH-Px activities of 8-week-old SeD-trial-2w group were almost non, while the normal group (fed on normal diet, MB-1) and SeC group (fed on SeD diet + 1.5 mg Se in drinking water) could keep GSH-Px activity. This SeD-trial-2w mouse model can be proposed for an H2O2-induced oxidative stress model for future experiments. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 19th biennial meeting for the Society for Free Radical Research International (SFRRI2018) | |||||
| 発表年月日 | ||||||
| 日付 | 2018-06-06 | |||||
| 日付タイプ | Issued | |||||
