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Radiobiology of sphere-forming rat mammary epithelial cells under nonadherent culture
https://repo.qst.go.jp/records/72348
https://repo.qst.go.jp/records/72348dc673727-8279-46ed-9a48-27261c01ffb1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-05-29 | |||||
| タイトル | ||||||
| タイトル | Radiobiology of sphere-forming rat mammary epithelial cells under nonadherent culture | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Imaoka, Tatsuhiko
× Imaoka, Tatsuhiko× Hosoki, Ayaka× Ogawa, Mari× Nishimura, Yukiko× Tani, Shusuke× Nishimura, Mayumi× Daino, Kazuhiro× Yamada, Yutaka× Kakinuma, Shizuko× Shimada, Yoshiya× 今岡 達彦× 細木 彩夏× 小川 真里× 西村 由希子× 谷 修祐× 西村 まゆみ× 臺野 和広× 山田 裕× 柿沼 志津子× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The mammary gland is highly susceptible to radiation carcinogenesis. It is vital to delineate the effect of radiation on tissue stem and progenitor cells, from which cancer cells arise, to understand the carcinogenic mechanisms of radiation. Herein we established a technique for the formation of ‘mammospheres’ of rat mammary epithelial cells (RMECs) using the nonadherent culturing method, which was previously developed for culturing human mammary progenitor cells, and examined the biological and radiobiological characteristics of mammosphere-forming cells. We found that mammospheres mainly consisted of proliferating cells that were double positive for cytokeratin (CK)14 and CK18 (markers for myoepithelial and luminal epithelial cells, respectively). Under differentiating conditions in adherent culture, mammospheres generated both CK14 and CK18 single-positive cells, implying that mammospheres consist of bipotent progenitor cells. Orthotropic transplantation assays showed that mammospheres contained lower stem cell activity than primary RMECs, suggesting that mammospheres are not enriched in stem cells. When primary RMECs were irradiated with up to 8 Gy in suspension and subjected to mammosphere formation, the efficiency of mammosphere formation, bromodeoxyuridine incorporation of cells therein, CK14/CK18 expression in mammospheres and their generation of single-positive cells in adherent culture were unaffected. These results suggest that mammosphere-forming cells of RMECs are resistant to radiation-induced proliferative death and perturbation of differentiation. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第15回幹細胞シンポジウム | |||||
| 発表年月日 | ||||||
| 日付 | 2017-05-27 | |||||
| 日付タイプ | Issued | |||||
