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Radiobiology of sphere-forming rat mammary epithelial cells under nonadherent culture

https://repo.qst.go.jp/records/72348
https://repo.qst.go.jp/records/72348
dc673727-8279-46ed-9a48-27261c01ffb1
Item type 会議発表用資料 / Presentation(1)
公開日 2017-05-29
タイトル
タイトル Radiobiology of sphere-forming rat mammary epithelial cells under nonadherent culture
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Imaoka, Tatsuhiko

× Imaoka, Tatsuhiko

WEKO 712599

Imaoka, Tatsuhiko

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Hosoki, Ayaka

× Hosoki, Ayaka

WEKO 712600

Hosoki, Ayaka

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Ogawa, Mari

× Ogawa, Mari

WEKO 712601

Ogawa, Mari

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Nishimura, Yukiko

× Nishimura, Yukiko

WEKO 712602

Nishimura, Yukiko

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Tani, Shusuke

× Tani, Shusuke

WEKO 712603

Tani, Shusuke

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Nishimura, Mayumi

× Nishimura, Mayumi

WEKO 712604

Nishimura, Mayumi

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Daino, Kazuhiro

× Daino, Kazuhiro

WEKO 712605

Daino, Kazuhiro

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Yamada, Yutaka

× Yamada, Yutaka

WEKO 712606

Yamada, Yutaka

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Kakinuma, Shizuko

× Kakinuma, Shizuko

WEKO 712607

Kakinuma, Shizuko

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Shimada, Yoshiya

× Shimada, Yoshiya

WEKO 712608

Shimada, Yoshiya

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今岡 達彦

× 今岡 達彦

WEKO 712609

en 今岡 達彦

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細木 彩夏

× 細木 彩夏

WEKO 712610

en 細木 彩夏

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小川 真里

× 小川 真里

WEKO 712611

en 小川 真里

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西村 由希子

× 西村 由希子

WEKO 712612

en 西村 由希子

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谷 修祐

× 谷 修祐

WEKO 712613

en 谷 修祐

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西村 まゆみ

× 西村 まゆみ

WEKO 712614

en 西村 まゆみ

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臺野 和広

× 臺野 和広

WEKO 712615

en 臺野 和広

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山田 裕

× 山田 裕

WEKO 712616

en 山田 裕

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柿沼 志津子

× 柿沼 志津子

WEKO 712617

en 柿沼 志津子

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島田 義也

× 島田 義也

WEKO 712618

en 島田 義也

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抄録
内容記述タイプ Abstract
内容記述 The mammary gland is highly susceptible to radiation carcinogenesis. It is vital to delineate the effect of radiation on tissue stem and progenitor cells, from which cancer cells arise, to understand the carcinogenic mechanisms of radiation. Herein we established a technique for the formation of ‘mammospheres’ of rat mammary epithelial cells (RMECs) using the nonadherent culturing method, which was previously developed for culturing human mammary progenitor cells, and examined the biological and radiobiological characteristics of mammosphere-forming cells. We found that mammospheres mainly consisted of proliferating cells that were double positive for cytokeratin (CK)14 and CK18 (markers for myoepithelial and luminal epithelial cells, respectively). Under differentiating conditions in adherent culture, mammospheres generated both CK14 and CK18 single-positive cells, implying that mammospheres consist of bipotent progenitor cells. Orthotropic transplantation assays showed that mammospheres contained lower stem cell activity than primary RMECs, suggesting that mammospheres are not enriched in stem cells. When primary RMECs were irradiated with up to 8 Gy in suspension and subjected to mammosphere formation, the efficiency of mammosphere formation, bromodeoxyuridine incorporation of cells therein, CK14/CK18 expression in mammospheres and their generation of single-positive cells in adherent culture were unaffected. These results suggest that mammosphere-forming cells of RMECs are resistant to radiation-induced proliferative death and perturbation of differentiation.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 第15回幹細胞シンポジウム
発表年月日
日付 2017-05-27
日付タイプ Issued
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