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Reversible DREADD inactivation of orbitofrontal cortex neurons in rhesus monkeys with contralateral rhinal cortex removal disrupts cued reward discrimination. I. Behavioral analysis.
https://repo.qst.go.jp/records/71741
https://repo.qst.go.jp/records/7174117a60ffd-cdcf-468f-a09f-d4b0bd0b6e7c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-06-25 | |||||
| タイトル | ||||||
| タイトル | Reversible DREADD inactivation of orbitofrontal cortex neurons in rhesus monkeys with contralateral rhinal cortex removal disrupts cued reward discrimination. I. Behavioral analysis. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Eldridge, Mark
× Eldridge, Mark× Lerchner, Walter× Minamimoto, Takafumi× Saunders, Richard× Richmond, Barry× 南本 敬史× サンダース リチャード× リッチモンド バリー |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Two monkeys received unilateral Rh aspiration lesions. They were then trained to perform a visually-cued reward size task. At the beginning of each trial, a visual cue signaled the amount of reward (1, 2, 4 or 8 drops) available for correctly detecting when a red visual target turned green. The reward size in any trial was picked at random, with equal probability. Although the identity of the cue was irrelevant to the successful completion of the red-green color discrimination, the error rates of the monkeys decreased with increasing drop size (GLMmixed; reward size, z = 12.74, p < 10¬-15). The operant demands were trial invariant, i.e., perform a sequential red-green color discrimination, so we interpret the differences in performance across reward size as reflecting the subjective valuation of the expected reward by the monkey as signaled by the cue. \nThe orbitofrontal cortex contralateral to the hemisphere with the rhinal cortex removal was injected with a lenti viral vector expressing a modified Gi-coupled receptor, hM4Di, (DREADD – Designer Receptor Activated by Designer Drug) that, when activated by systemically delivered clozapine-N-oxide (CNO), causes neuronal silencing (see accompanying poster). If effective, activation with CNO should lead to a functional disconnection of Rh from OFC. \nAfter completion of all surgical procedures, the behavior of the monkeys was indistinguishable from normal monkeys or these monkeys before the DREADD injections. In behavioral testing sessions begun with systemic injection of CNO (4-5 repetitions), there was a marked reduction in the discrimination between expected reward sizes, and an overall reduction in error rate for both monkeys (GLMmixed; Monkey D: CNO*reward_size, p < 10-3, CNO, p < 10-5; Monkey S: CNO*reward_size, p < 0.005, CNO, p < 10-11). These results demonstrate that the CNO-DREADD system can be effective for altering behavior when applied to old world monkey cortex. The reward deficit seen after a Rh-OFC disconnection could be due to an inability to act on already learned reward relationships – the monkeys are only impaired on the days with CNO treatment, whereas they respond normally to cues predicting forthcoming rewards on the days before and after the CNO treatment. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Society for Neuroscience | |||||
| 発表年月日 | ||||||
| 日付 | 2014-11-17 | |||||
| 日付タイプ | Issued | |||||
