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Activation of Nrf2 Antioxidative Response In Normal Human Lung Fibroblast WI38 By Cytoplasm Targeted Irradiation With Proton Microbeam In NIRS
https://repo.qst.go.jp/records/71715
https://repo.qst.go.jp/records/717155a0214ad-3ff0-4ead-bf07-9693fd3d29f7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-06-11 | |||||
| タイトル | ||||||
| タイトル | Activation of Nrf2 Antioxidative Response In Normal Human Lung Fibroblast WI38 By Cytoplasm Targeted Irradiation With Proton Microbeam In NIRS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Wang, Jun
× Wang, Jun× Konishi, Teruaki× Kobayashi, Alisa× Oikawa, Masakazu× K., Hei Tom× Uchihori, Yukio× Wu, Lijun× 小西 輝昭× 小林 亜利紗× 及川 将一× 内堀 幸夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It has also been reported that extra-nuclear irradiation resembles biological effects of nuclear-traversed irradiation and production of free radical species is a key mediator of the process. Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important regulator of cellular oxidative status. It remains unknown whether the Nrf2 will be activated in cells exposed to cytoplasm targeted irradiation. To this point, a study with normal human lung fibroblast WI38 on Single Particle Irradiation System to Cells (SPICE) facility at NIRS was performed. The results showed that cytoplasm targeted radiation with proton promoted the nuclear transfer of Nrf2. A dose-dependent manner of Nrf2 nuclear transfer was observed within the dose range from 0 to 1000 protons. By measuring the fraction of Nrf2 in nucleus, it was found that upon cytoplasm targeted with 500 protons, the accumulation of Nrf2 in nucleus enhanced with the extension of post-radiation time. The nuclear transfer of Nrf2 after cytoplasm targeted was controlled by activated MAPK ERK1/2, which was shown by the facts that the phosphorylation of MAPK ERK1/2 in WI38 increased after radiation, and the application of MAPK ERK1/2 inhibitor U0126 blocked the nuclear transfer of Nrf2. We further investigated the effect of activation of Nrf2 on radiation induced DNA damage and the results showed that pre-activation of Nrf2 with chemical inducer will alleviated DNA double strand breaks caused by cytoplasm targeted radiation. In conclusion, the current work indicated that Nrf2 antioxidative response was activated after cytoplasm targeted radiation and this might play a protective role against radiation induced DNA damage. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 12th International Workshop on Microbeam Probes of Cellular Radiation Response | |||||
| 発表年月日 | ||||||
| 日付 | 2015-06-01 | |||||
| 日付タイプ | Issued | |||||
