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Transmission and repair of DNA damage signal to bystander cells from the population of proton microbeam irradiated human cells
https://repo.qst.go.jp/records/71710
https://repo.qst.go.jp/records/717108f31af30-0e0e-4495-8ca0-ffae1334ecfc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-06-05 | |||||
| タイトル | ||||||
| タイトル | Transmission and repair of DNA damage signal to bystander cells from the population of proton microbeam irradiated human cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
N., Pandey Bandri
× N., Pandey Bandri× Desai, Sejal× Kobayashi, Alisa× Konishi, Teruaki× 小林 亜利紗× 小西 輝昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Microbeam provides important tool to study radiation induced bystander effect with precise localization of target cells in a population delivering defined radiation dose/number of particles. Microbeam technique has been employed to investigate radiation effects to bystander cells using various biological end points. However, studies are limited in literature revealing transmission and repair of DNA damage signal from microbeam irradiated cells to bystander cells. In present study, we have studied direct and bystander damage in proton microbeam irradiated human lung cancer (A549) and normal lung fibroblasts (WI38) cells using SPICE microbeam facility (3.4 MeV; ~2 M beam diameter; LET 11.7 KeV/M) at NIRS, Japan. A549 or WI38 cells were irradiated with increasing number of protons (10-200 protons/cell) followed by measurement of -H2AX foci. -H2AX foci were observed in A549 cells irradiated with ≥50 protons/cell, which were observed at ≥200 protons/cell in WI38 cells. These results suggest requirement of higher number of protons to detect proton microbeam induced DSBs by -H2AX foci in WI38 than in A549 cells. Repair kinetics of DSBs induced by proton microbeam was faster in WI38 than A549 cells. To evaluate the bystander effect in proton irradiated A549 cells, -H2AX foci were analyzed in irradiated area of cells as well as three adjacent side-by-side areas of non-irradiated (bystander) cells on the irradiation dish. When images were analyzed for -H2AX intensity, a pattern was observed, which showed maximum DNA damage in directly irradiated cells after 30 min of irradiation. Measurement at subsequent incubation periods showed transmission of damaging signal in adjacent areas and distance travelled of damaging signal was dependent on incubation period. Our results provide novel understanding of transmission and repair signal from proton microbeam irradiated cells to bystander cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 12th International Workshop on Microbeam Probes of Cellular Radiation Response | |||||
| 発表年月日 | ||||||
| 日付 | 2015-05-30 | |||||
| 日付タイプ | Issued | |||||
