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In vivo analysis of processing bodies using transgenic mice expressing GFP-Dcp1a
https://repo.qst.go.jp/records/71696
https://repo.qst.go.jp/records/7169658aa5cf6-7816-4f7c-b9b4-09ef3ce52898
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-06-03 | |||||
| タイトル | ||||||
| タイトル | In vivo analysis of processing bodies using transgenic mice expressing GFP-Dcp1a | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
塚本, 智史
× 塚本, 智史× 原, 太一× 佐藤, 健× 南, 直治郎× 鬼頭, 靖司× 小久保, 年章× 塚本 智史× 南 直治郎× 鬼頭 靖司× 小久保 年章 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The control of mRNA translation and degradation is important in the regulation of eukaryotic gene expression. Processing bodies (P-bodies) are cytoplasmic foci where mRNA decapping occurs and are therefore closely linked to mRNA degradation, storage, and translation. Although it is known that the number and size of P-bodies change in response to cellular stress including cell cycle arrest, DNA damage and senescence, their physiological functions are largely nknown. In this study,we generated transgenic mice that ubiquitously expressed GFP fused with the decapping enzyme Dcp1a under the control of the CAG promoter. GFP-Dcp1a was expressed in almost all tissues examined, with higher levels detected in heart, muscle, and testis. Immunofluorescence analysis in frozen tissue sections revealed the co-localization of GFP-Dcp1a and EDC4, an endogenous P-body marker. We also confirmed the loss of P-bodies by cycloheximide treatment of embryonic fibroblasts isolated from the transgenic mice. We further analyzed the changes in P-bodies during heart development and found that their numbers dramatically increased after birth and then de-creased in a time-dependent manner. Taken together, our results show that this transgenic mouse provides a tool for investigating the physiological functions of mammalian P-bodies in a wide range of research field. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 15th International Congress of Radiation Research (ICRR 2015) | |||||
| 発表年月日 | ||||||
| 日付 | 2015-05-28 | |||||
| 日付タイプ | Issued | |||||
