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  1. 学会発表・講演等
  2. ポスター発表

In-vivo Imaging of Neuronal Differentiation and Function of Intracranially Implanted Induced Pluripotent Stem Cells (iPSCs) Using A Designer Receptor Exclusively Activated by A Designer Drug (DREADD)

https://repo.qst.go.jp/records/71549
https://repo.qst.go.jp/records/71549
a310bfed-f68b-4388-9ad1-a639571d10e7
Item type 会議発表用資料 / Presentation(1)
公開日 2014-11-25
タイトル
タイトル In-vivo Imaging of Neuronal Differentiation and Function of Intracranially Implanted Induced Pluripotent Stem Cells (iPSCs) Using A Designer Receptor Exclusively Activated by A Designer Drug (DREADD)
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Ji, Bin

× Ji, Bin

WEKO 838957

Ji, Bin

Search repository
Kaneko, Hiroyuki

× Kaneko, Hiroyuki

WEKO 838958

Kaneko, Hiroyuki

Search repository
Minamimoto, Takafumi

× Minamimoto, Takafumi

WEKO 838959

Minamimoto, Takafumi

Search repository
Inoue, Haruhisa

× Inoue, Haruhisa

WEKO 838960

Inoue, Haruhisa

Search repository
Takeuchi, Hiroki

× Takeuchi, Hiroki

WEKO 838961

Takeuchi, Hiroki

Search repository
Kumata, Katsushi

× Kumata, Katsushi

WEKO 838962

Kumata, Katsushi

Search repository
Ming-Rong, Zhang

× Ming-Rong, Zhang

WEKO 838963

Ming-Rong, Zhang

Search repository
Aoki, Ichio

× Aoki, Ichio

WEKO 838964

Aoki, Ichio

Search repository
Seki, Chie

× Seki, Chie

WEKO 838965

Seki, Chie

Search repository
Ono, Maiko

× Ono, Maiko

WEKO 838966

Ono, Maiko

Search repository
Tokunaga, Masaki

× Tokunaga, Masaki

WEKO 838967

Tokunaga, Masaki

Search repository
Tsukamoto, Satoshi

× Tsukamoto, Satoshi

WEKO 838968

Tsukamoto, Satoshi

Search repository
Tanabe, Koji

× Tanabe, Koji

WEKO 838969

Tanabe, Koji

Search repository
Takahashi, Kazutoshi

× Takahashi, Kazutoshi

WEKO 838970

Takahashi, Kazutoshi

Search repository
Minamihisamatsu, Takeharu

× Minamihisamatsu, Takeharu

WEKO 838971

Minamihisamatsu, Takeharu

Search repository
Suhara, Tetsuya

× Suhara, Tetsuya

WEKO 838972

Suhara, Tetsuya

Search repository
Higuchi, Makoto

× Higuchi, Makoto

WEKO 838973

Higuchi, Makoto

Search repository
Ji, Bin

× Ji, Bin

WEKO 838974

en Ji, Bin

Search repository
Minamimoto, Takafumi

× Minamimoto, Takafumi

WEKO 838975

en Minamimoto, Takafumi

Search repository
Kumata, Katsushi

× Kumata, Katsushi

WEKO 838976

en Kumata, Katsushi

Search repository
Ming-Rong, Zhang

× Ming-Rong, Zhang

WEKO 838977

en Ming-Rong, Zhang

Search repository
Aoki, Ichio

× Aoki, Ichio

WEKO 838978

en Aoki, Ichio

Search repository
Seki, Chie

× Seki, Chie

WEKO 838979

en Seki, Chie

Search repository
Ono, Maiko

× Ono, Maiko

WEKO 838980

en Ono, Maiko

Search repository
Tokunaga, Masaki

× Tokunaga, Masaki

WEKO 838981

en Tokunaga, Masaki

Search repository
Tsukamoto, Satoshi

× Tsukamoto, Satoshi

WEKO 838982

en Tsukamoto, Satoshi

Search repository
Minamihisamatsu, Takeharu

× Minamihisamatsu, Takeharu

WEKO 838983

en Minamihisamatsu, Takeharu

Search repository
Suhara, Tetsuya

× Suhara, Tetsuya

WEKO 838984

en Suhara, Tetsuya

Search repository
Higuchi, Makoto

× Higuchi, Makoto

WEKO 838985

en Higuchi, Makoto

Search repository
抄録
内容記述タイプ Abstract
内容記述 Induced pluripotent stem cells (iPSCs) provide a promising resource for cell replacement therapy in neurological diseases. In the present study, we have applied a designer receptor exclusively activated by a designer drug (DREADD) derived from human M4 muscarinic acetylcholine receptor (hM4D) and its synthetic ligand to in-vivo visualization of neuronal differentiation and function of iPSC-derived grafts implanted into the brain. We successfully captured expression of hM4D driven by neuron-specific Thy-1 promoter in newly-developed hM4D transgenic (hM4D Tg) mice with a 11C-labeled positron emission tomography (PET) ligand for hM4D. We also established iPSCs from a hM4D Tg mouse (hM4D-iPSC), and visualized time course of neuronal differentiation of grafts generated from these iPSCs in the living wild-type mouse brain by longitudinal PET imaging of hM4D with its specific radioligand. Quantitative assessment for cerebral blood flow using arterial spin labeling (ASL) MRI indicated suppression of neuronal activity by clozapine-N-oxide (CNO), an exclusive activator of hM4D, in hM4D Tg but not wild-type mice, in consistency with attenuation of locomotion behaviors. Furthermore, we found CNO-induced reduction of cerebral blood flow in areas associated with implantation of hM4D-iPSC-derived grafts by ASL-MRI of recipient mice. Our results support the utility of hM4D in combination with PET and ASL-MRI for in-vivo longitudinal monitoring of neuronal differentiation and functional manipulation of iPSC-derived implants in the brain. Since this technology is potentially applicable to humans, it would accelerate translational research and development of cell replacement therapy towards clinical trials.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 Neuroscience meeting 2014
発表年月日
日付 2014-11-21
日付タイプ Issued
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