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2,2-Diphenyl-1-picrylhydrazyl Radical Solubilized by beta-Cyclodextrin in Water for Evaluation of Radical-Scavenging Activity of Antioxidants in Aqueous Systems
https://repo.qst.go.jp/records/71533
https://repo.qst.go.jp/records/715332eb43d19-e6ce-4dfa-923a-95226cd996f3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-06 | |||||
| タイトル | ||||||
| タイトル | 2,2-Diphenyl-1-picrylhydrazyl Radical Solubilized by beta-Cyclodextrin in Water for Evaluation of Radical-Scavenging Activity of Antioxidants in Aqueous Systems | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
中西, 郁夫
× 中西, 郁夫× 今井, 耕平× 福原, 潔× 小澤, 俊彦× 松本, 謙一郎× 中西 郁夫× 今井 耕平× 小澤 俊彦× 松本 謙一郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical is a relatively stable radical and frequently used as a model of reactive oxygen species to evaluate the radical-scafenging activity of antioxidants. However, the poor solubility of DPPH radical to water has precluded its use in aqueous systems. In this study, we used beta-cyclodextrin (CD) to solubilize DPPH radical in water. Ball milling of the mixture containing DPPH radical and beta-CD for 1 h, followed by an addition of water and filtration with a membrane filter gave a purple solution. This solution showed an absorption band at 525 nm, which is diagnostic of DPPH radical. When ascorbic acid was added to the solution, the band at 525 nm disappeared immediately. This indicates that ascorbic acid efficiently scavenge DPPH radical in water. Thus, DPPH radical solubilized by beta-CD in water may be a powerful tool to evaluate antioxidative activity of antioxidants in aqueous systems. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SFRBM's 21st Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2014-11-22 | |||||
| 日付タイプ | Issued | |||||
