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Zr-89 immuno-PET imaging targeting HER3 expression in mouse models
https://repo.qst.go.jp/records/71518
https://repo.qst.go.jp/records/715187b8bebab-d3f0-418d-925b-1fbf6e9639ef
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-10-09 | |||||
| タイトル | ||||||
| タイトル | Zr-89 immuno-PET imaging targeting HER3 expression in mouse models | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yuan, Qinghua
× Yuan, Qinghua× Furukawa, Takako× Jin, Zhao-Hui× U, Winn Aung× Yoshii, Yukie× Sugyou, Aya× Nagatsu, Kotaro× Endo, Hiroko× Inoue, Masahiro× Masuko, Takashi× Fujibayashi, Yasuhisa× Saga, Tsuneo× 元 清華× 古川 高子× 金 朝暉× Ｕ Ｗｉｎｎ Ａｕｎｇ× 吉井 幸恵× 須尭 綾× 永津 弘太郎× 藤林 康久× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cancer Tissue-Originated Spheroid (CTOS) is a recently developed tissue culture method, in which the properties of original tumors are preserved through maintaining the cell-cell contact (1). CTOS provides a unique model for cancer research and the characterization of CTOSs have revealed that HER3 signaling plays important role in the growth and remodeling of CTOSs, the process related to the malignant properties of cancer (unpublished data). Postulating HER3 as a target reflecting cancer malignancy, we explored the possibility of in vivo imaging of HER3 expression using anti-HER3 antibody. An anti-HER3 monoclonal antibody (Mab#58) was conjugated with desferrioxamine (DF) using p-isothiocyanatobenzyl-desferrioxamine and labeled with Zr-89 produced in our institute, following the previously reported protocol (2). The number of DF attached to one IgG molecule was 2.3 in average, and the immunoactivity was preserved through the conjugation, confirmed by cell binding assay. The radiochemical purity after the purification was over 90 %, and the specific radioactivity was 40-110 MBq/mg. Biodistribution study of the Zr-89-labeled antibody in mice bearing both HER3 overexpressing Her3/RH7777 and the parent RH7777 xenografts on the back showed that in most of the tissues examined, including RH7777 xenograft, radioactivity in the tissue (%D/g) decreased or unchanged from day1 to day6 after the injection of the labeled antibody, while that in Her3/RH7777 xenograft increased from day1 to day4, reaching 12.2 %D/g. The radioactivity in Her3/RH7777 xenograft was significantly higher than that in RH7777 xenograft through day1 to day6, indicating specific accumulation of the labeled antibody. Her3/RH7777 xenograft was visualized by a small animal PET, with the accumulation apparently higher than that in RH7777 xenograft, from day1 to day6. The xenografts of colon and lung CTOSs were also clearly visualized by small animal PET. The successful imaging demonstrated the feasibility of PET imaging of HER3 expressing tumors by Zr-89 labeled anti-HER3 antibody, which could lead to imaging of a malignant characteristic of cancer. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The World Molecular Imaging Congress 2014 | |||||
| 発表年月日 | ||||||
| 日付 | 2014-09-17 | |||||
| 日付タイプ | Issued | |||||
