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Impaired cerebrovascular responses to sensory stimulation and hypercapnia induced by vascular amyloid in a mouse model of alzheimer’s disease amyloidosis
https://repo.qst.go.jp/records/71458
https://repo.qst.go.jp/records/71458401e49ef-1949-4d22-a7e2-256de2b6ff27
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-06-02 | |||||
| タイトル | ||||||
| タイトル | Impaired cerebrovascular responses to sensory stimulation and hypercapnia induced by vascular amyloid in a mouse model of alzheimer’s disease amyloidosis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
田桑, 弘之
× 田桑, 弘之× et.al× 田桑 弘之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: Cerebrovascular dysfunction has been implicated in Alzheimer’s disease (AD). To explore the mechanistic links between cerebrovascular dysfunction and the pathogenesis of AD, we previously performed laser-Doppler flowmetry (LDF) measurement of cerebrovascular responses to whisker stimulation in amyloid precursor protein transgenic (APP23) mice and wild-type (WT) mice in awake condition. The results showed that cerebrovascular dysfunction occured along with disposition of parenchymal and vascular amyloid in the brain. In this study, we investigated effects of vascular amyloid on cerebrovascular responses to sensory stimulation and hypercapnia using two-photon microscopy. Methods: Two-photon microscopic imaging was performed on APP23 mice (N=4), which exhibited abundant parenchymal and vascular amyloid, and WT mice (N=4) aged at 23 months in awake condition. Amyloid lesions and microvessels were fluorescently labeled with an amyloid-binding probe (PBB2)1) and sulforhodamine 101, respectively. Changes in diameter of arterioles were measured during whisker stimulation and hypercapnia. Results: In APP23 mice, the percentage changes in diameter of arterioles with vascular amyloid during sensory stimulation and hypercapnia were 1.7 ± 1.7% and 1.4 ± 0.7% (mean ± SD), respectively. By contrast, the percentage changes in diameter of arterioles without amyloid during sensory stimulation and hypercapnia being 22.8 ± 9.0% and 25.1 ± 3.3%, respectively (Fig. 4A). The percentage changes in diameter of arterioles during sensory stimulation and hypercapnia in WT mice were 21.5 ± 3.0% and 24.0 ± 3.9%, respectively. Conclusion: These indicate that attenuation of vasodilation can be observed only in arterioles covered with vascular amyloid, while arterioles without vascular amyloid can respond normally. The vascular amyloid, but not the parenchymal amyloid may be critically implicated in the cerebrovascular dysfunction induced by AD-type cerebral amyloidosis. \nReference: 1) Maruyama M et al., 2013, Neuron 79:1094-1108. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | NRM2014にてポスター発表を行う。 | |||||
| 発表年月日 | ||||||
| 日付 | 2014-06-22 | |||||
| 日付タイプ | Issued | |||||
