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Mitotic-spindle associated genes are among the common genes responding to IR
https://repo.qst.go.jp/records/71417
https://repo.qst.go.jp/records/714171284abaf-d0f0-476c-857c-b303a680a60d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-02-14 | |||||
| タイトル | ||||||
| タイトル | Mitotic-spindle associated genes are among the common genes responding to IR | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
藤森, 亮
× 藤森, 亮× 平川, 博一× et.al× 藤森 亮× 平川 博一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Accelerated ion particle with highLET1 is promising for the treatment of certain kinds of malignancy. Molecular basis of its advantage over conventional radiotherapy, however, has not been clarified. Using HiCEP2, we compared gene expression profiles in the normal diploid human fibroblasts (HFLIII) irradiated with three types of clinically relevant radiation at 2Gy; X-ray (0.9 Gy/min), and carbon ion beam of either low (13 keV/μm) or high (70 keV/μm) LET by HIMAC3. RNA was extracted at 2, 4 and 6 hr later and the expression levels for approx.16,000 transcripts were compared between samples irradiated and mock-irradiated. Through all the nine comparison analyses, we selected eleven IR-responsive genes (signatures), which included upregulated transcripts (BTG2, ATF3, CDKN1A etc.) and also ‘downregulated’ genes, CCNF4 and ASPM5. Further comparison study demonstrated that carbon particles of higher LET prolong the expression of earlier-responding genes particularly among those which are known to be upregulated under p53 control. Since it was documented that both CCNF and ASPM are required for normal mitotic-spindle formation in somatic cells, our present results would provide a novel insight into the carbon ion beams causing the significant reduction in these spindle-mitotic proteins on DNA damage response. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2011-08-30 | |||||
| 日付タイプ | Issued | |||||
