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We previously reported suppression of red blood cell (RBC) velocity flowing in capillaries conversely in the region of neuronal activation (1,2). To further understand the mechanisms of CBF control during CSD, we examined temporal pattern of diametric changes in pial vessels and CBF. \nMethods: To visualize blood vessels, we used Tie2-GFP transgenic mice (N=16), in which specifically vascular endothelial cells emit fluorescence. Under urethane anesthesia and artificial ventilation, a cranial window was installed on the temporo-parietal region of the cerebral cortex. KCl (1 M) was applied on the brain surface through a burr hole posterior to the cranial window to elicit CSD. Diameters of pial arteries, veins and capillaries were measured with the confocal laser-scanning fluorescence microscopy and an image analysis software ImagePro. DC potential was simultaneously recorded next to the window along with CBF by laser Doppler flowmeter. The fluorecent flow in cerebral vessels was recorded on a video after the intravenous bolus injection before and after CSD elicitation. Pixel-by-pixel microflow values (a reciprocal of mean transit time: 1/MTT) were calculated from the temporal intensity change by the in-house Matlab software KEIO-IS1 in the same region of interest (3). \nResults: Application of KCl induced biphasic response, first constriction of pial arteries (-35.4pm18.8%) during DC potential deflection and then dilation (5.2pm12.6%). CBF increased with transient drop observed at the peak time of vasoconstriction.There was no significant correlation between baseline diameters of the pial arteries and their % changes during CSD (r=0.342). Pial veins and capillaries showed small but significant dilation. Second and third waves of CSD also elicited vasodilation but the response was diminished (4.8pm10.9% and 1.9pm7.4%, respectively), whereas CBF increase (29.6pm14.5% and 27.7pm12.0%) without transient drop was repeatedly observed without diminution. Mild vasoconstriction was observed only in limited cases (n=25/40 and 10/30). After CSD, CBF decreased and reached a plateau below the baseline level (-19.7 pm 9.2%) (post-CSD oligemia), whereas 1/MTT decreased only 12.4pm11.6% and became stable with diameter of pial vessels returning to the baseline levels.\nConclusion: Initial increase in CBF during first CSD may be counteracted with vasoconstriction induced through mechanisms different from flow-metabolism coupling. Diametric responses in pial vessels were diminished, whereas increase in CBF was repeatedly observed. Thus, cerebrovascular control in response to CSD might be largely affected by peripheral vessels from the pial artery such as penetrating arteries. Together with our previous reports of suppressed RBC flow (1,2), it is suggested that diameter of pial artery and cortical CBF are controlled differently during CSD.\nReferences: \n(1) Unekawa M et al, Microcirculation, 19:166-74, 2012. \n(2) Unekawa M et al, J Neurosci Res, in press. \n(3) Tomita Y et al, Brain Res, 1372:59-69, 2011.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "Brain \u0026 BrainPET 2013", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Unekawa, Miyuki"}], "nameIdentifiers": [{"nameIdentifier": "701721", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tomita, Yutaka"}], "nameIdentifiers": [{"nameIdentifier": "701722", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Toriumi, Haruki"}], "nameIdentifiers": [{"nameIdentifier": "701723", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Osada, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "701724", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Masamoto, Kazuto"}], "nameIdentifiers": [{"nameIdentifier": "701725", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawaguchi, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "701726", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Yoshiaki"}], "nameIdentifiers": [{"nameIdentifier": "701727", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanno, Iwao"}], "nameIdentifiers": [{"nameIdentifier": "701728", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Norihiro"}], "nameIdentifiers": [{"nameIdentifier": "701729", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "畝川 美悠紀", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701730", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "冨田 裕", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701731", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鳥海 春樹", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701732", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "長田 高志", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701733", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "正本 和人", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701734", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "川口 拓之", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701735", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "菅野 巖", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701736", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "DIFFERENT RESPONSE IN DIAMETER OF PIAL VESSELS AND IN CEREBRAL BLOOD FLOW ASSOCIATED WITH CORTICAL SPREADING DEPRESSION IN ANESTHETIZED MICE", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "DIFFERENT RESPONSE IN DIAMETER OF PIAL VESSELS AND IN CEREBRAL BLOOD FLOW ASSOCIATED WITH CORTICAL SPREADING DEPRESSION IN ANESTHETIZED MICE"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/71366", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-11-18"}, "publish_date": "2013-11-18", "publish_status": "0", "recid": "71366", "relation": {}, "relation_version_is_last": true, "title": ["DIFFERENT RESPONSE IN DIAMETER OF PIAL VESSELS AND IN CEREBRAL BLOOD FLOW ASSOCIATED WITH CORTICAL SPREADING DEPRESSION IN ANESTHETIZED MICE"], "weko_shared_id": -1}
  1. 学会発表・講演等
  2. ポスター発表

DIFFERENT RESPONSE IN DIAMETER OF PIAL VESSELS AND IN CEREBRAL BLOOD FLOW ASSOCIATED WITH CORTICAL SPREADING DEPRESSION IN ANESTHETIZED MICE

https://repo.qst.go.jp/records/71366
https://repo.qst.go.jp/records/71366
48930291-7139-48a0-afd7-cd18a1caee16
Item type 会議発表用資料 / Presentation(1)
公開日 2013-11-18
タイトル
タイトル DIFFERENT RESPONSE IN DIAMETER OF PIAL VESSELS AND IN CEREBRAL BLOOD FLOW ASSOCIATED WITH CORTICAL SPREADING DEPRESSION IN ANESTHETIZED MICE
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Unekawa, Miyuki

× Unekawa, Miyuki

WEKO 701721

Unekawa, Miyuki

Search repository
Tomita, Yutaka

× Tomita, Yutaka

WEKO 701722

Tomita, Yutaka

Search repository
Toriumi, Haruki

× Toriumi, Haruki

WEKO 701723

Toriumi, Haruki

Search repository
Osada, Takashi

× Osada, Takashi

WEKO 701724

Osada, Takashi

Search repository
Masamoto, Kazuto

× Masamoto, Kazuto

WEKO 701725

Masamoto, Kazuto

Search repository
Kawaguchi, Hiroshi

× Kawaguchi, Hiroshi

WEKO 701726

Kawaguchi, Hiroshi

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Itoh, Yoshiaki

× Itoh, Yoshiaki

WEKO 701727

Itoh, Yoshiaki

Search repository
Kanno, Iwao

× Kanno, Iwao

WEKO 701728

Kanno, Iwao

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Suzuki, Norihiro

× Suzuki, Norihiro

WEKO 701729

Suzuki, Norihiro

Search repository
畝川 美悠紀

× 畝川 美悠紀

WEKO 701730

en 畝川 美悠紀

Search repository
冨田 裕

× 冨田 裕

WEKO 701731

en 冨田 裕

Search repository
鳥海 春樹

× 鳥海 春樹

WEKO 701732

en 鳥海 春樹

Search repository
長田 高志

× 長田 高志

WEKO 701733

en 長田 高志

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正本 和人

× 正本 和人

WEKO 701734

en 正本 和人

Search repository
川口 拓之

× 川口 拓之

WEKO 701735

en 川口 拓之

Search repository
菅野 巖

× 菅野 巖

WEKO 701736

en 菅野 巖

Search repository
抄録
内容記述タイプ Abstract
内容記述 Objectives: Cortical spreading depression (CSD), a propagating phenomenon of depolarizing neurons and astroglia, dramatically affects cortical cerebral blood flow (CBF) by still undetermined mechanisms. We previously reported suppression of red blood cell (RBC) velocity flowing in capillaries conversely in the region of neuronal activation (1,2). To further understand the mechanisms of CBF control during CSD, we examined temporal pattern of diametric changes in pial vessels and CBF.
Methods: To visualize blood vessels, we used Tie2-GFP transgenic mice (N=16), in which specifically vascular endothelial cells emit fluorescence. Under urethane anesthesia and artificial ventilation, a cranial window was installed on the temporo-parietal region of the cerebral cortex. KCl (1 M) was applied on the brain surface through a burr hole posterior to the cranial window to elicit CSD. Diameters of pial arteries, veins and capillaries were measured with the confocal laser-scanning fluorescence microscopy and an image analysis software ImagePro. DC potential was simultaneously recorded next to the window along with CBF by laser Doppler flowmeter. The fluorecent flow in cerebral vessels was recorded on a video after the intravenous bolus injection before and after CSD elicitation. Pixel-by-pixel microflow values (a reciprocal of mean transit time: 1/MTT) were calculated from the temporal intensity change by the in-house Matlab software KEIO-IS1 in the same region of interest (3).
Results: Application of KCl induced biphasic response, first constriction of pial arteries (-35.4pm18.8%) during DC potential deflection and then dilation (5.2pm12.6%). CBF increased with transient drop observed at the peak time of vasoconstriction.There was no significant correlation between baseline diameters of the pial arteries and their % changes during CSD (r=0.342). Pial veins and capillaries showed small but significant dilation. Second and third waves of CSD also elicited vasodilation but the response was diminished (4.8pm10.9% and 1.9pm7.4%, respectively), whereas CBF increase (29.6pm14.5% and 27.7pm12.0%) without transient drop was repeatedly observed without diminution. Mild vasoconstriction was observed only in limited cases (n=25/40 and 10/30). After CSD, CBF decreased and reached a plateau below the baseline level (-19.7 pm 9.2%) (post-CSD oligemia), whereas 1/MTT decreased only 12.4pm11.6% and became stable with diameter of pial vessels returning to the baseline levels.
Conclusion: Initial increase in CBF during first CSD may be counteracted with vasoconstriction induced through mechanisms different from flow-metabolism coupling. Diametric responses in pial vessels were diminished, whereas increase in CBF was repeatedly observed. Thus, cerebrovascular control in response to CSD might be largely affected by peripheral vessels from the pial artery such as penetrating arteries. Together with our previous reports of suppressed RBC flow (1,2), it is suggested that diameter of pial artery and cortical CBF are controlled differently during CSD.
References:
(1) Unekawa M et al, Microcirculation, 19:166-74, 2012.
(2) Unekawa M et al, J Neurosci Res, in press.
(3) Tomita Y et al, Brain Res, 1372:59-69, 2011.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 Brain & BrainPET 2013
発表年月日
日付 2013-05-23
日付タイプ Issued
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