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High-Fat Diet Induces Changes in DNA Methylatiion and microRNA Reguations After Exposure to Ionizing Radiation
https://repo.qst.go.jp/records/71313
https://repo.qst.go.jp/records/7131399468d7c-5fbb-4e78-a96c-daa0bc7c8381
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-25 | |||||
| タイトル | ||||||
| タイトル | High-Fat Diet Induces Changes in DNA Methylatiion and microRNA Reguations After Exposure to Ionizing Radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Vares, Guillaume
× Vares, Guillaume× Nakajima, Tetsuo× Bing, Wang× Ishii, Hiroko× Nenoi, Mitsuru× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 中島 徹夫× 王 冰× 石井 洋子× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Obesity has been associated with various pathologies including cancer. It is estimated that up to 7% of all new cancers in US were due to obesity. Although some relationships were described between diet and ionizing radiation, there is still much to know about the dietary influences on radiation risks. In order to evaluate the modulation of radiation responses according to calorie intake, we exposed C3H and C57BL/6 mice fed a normal diet or a high-fat (HF) diet to fractionated doses of X-rays. Bone marrow micronucleus assays suggested that HF diet did not modulate radiation-induced genotoxicity. Both HF diet and irradiation resulted in increased oxidative damage, H2AX levels and proliferation in C57BL/6 mouse liver sections. Using methylation-specific PCR, we observed that the promoters of p16 and Dapk genes were methylated only in the livers of C57BL/6 mice fed a HF diet (irradiated and non-irradiated); Mgmt promoter was methylated in irradiated and/or HF diet-fed mice. In addition, methylation PCR arrays identified Ep300 and Socs1 (whose promoters exhibited higher methylation levels in non-irradiated HF diet-fed mice) as potential targets for further studies. We then compared microRNA regulations after radiation exposure in the livers of C57BL/6 mice fed a normal or an HF diet, using microRNA arrays. Interestingly, radiation-specific microRNA regulations observed in normal mice were not observed in obese mice, fed a HF diet. All together, our results suggested the existence of dietary effects on radiation responses in mice. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第56回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-10-20 | |||||
| 日付タイプ | Issued | |||||
