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Effects of Carbon Ion Beam Alone or in Combination with Sorafenib on Putative Liver Cancer Stem Cells
https://repo.qst.go.jp/records/71286
https://repo.qst.go.jp/records/71286da9c2c11-bf5d-422d-9f60-244e4668eb6f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-10 | |||||
| タイトル | ||||||
| タイトル | Effects of Carbon Ion Beam Alone or in Combination with Sorafenib on Putative Liver Cancer Stem Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Wakai, Toshifumi× Shirai, Toshiyuki× Kamada, Tadashi× 崔 星× 白井 敏之× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To investigate whether carbon ion beam alone or in combination with sorafenib has beneficial effects compared to X-ray by targeting putative cancer stem cells. Methods: Human liver cancer stem-like cells sorted from HepG2 and Huh7 cells were treated with carbon ion or X-ray irradiation alone or in combination with sorafenib, and then colony and spheroid formation assay, apoptosis assay, γH2AX foci formation assays were performed. Histopathological changes of xenograft tumors after carbon or X-ray irradiation was also examined. Results: CD133+/CD90+ cells significantly have higher number of colony and spheroid compared to CD133-/CD90- cells. CD133+/CD90+ cells have shown more potential to form tumors in SCID mice than CD133-/CD90- cells. Colony assay showed that CD133+/CD90+ cells appeared to be radioresistant to both X-ray and carbon ion beam, but carbon beam, especially carbon ion combined with sorafenib remarkably destroyed those of cancer stem-like cells. The percentage of apoptotic cells as well as the number of γH2AX foci in the CD133+/CD90+ cells was significantly lager after carbon ion beam compared to X-ray irradiation, and it was more increased when combined with sorafenib. Carbon ion irradiation with 15 Gy induced more severe xenograft tumor cell cavitation and fibrosis compared with 30 Gy X-rays, and tumors were significantly regressed following 30 Gy carbon ion irradiation. Conclusion: Taken together, carbon ion beam combined with sorafenib has more potential to kill CD133+/CD90+ cancer stem-like cells at relatively lower doses via unrepairable DNA damage in vitro as well as tumor regression in vivo compared to X-ray |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 17th ECCO, 38th ESMO, 32nd ESTRO European Cancer Congress, | |||||
| 発表年月日 | ||||||
| 日付 | 2013-10-01 | |||||
| 日付タイプ | Issued | |||||
