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What we know about the communication between carbon-irradiated cancer cells and bystander human fibroblasts
https://repo.qst.go.jp/records/71270
https://repo.qst.go.jp/records/712701d692af2-100c-4baf-876d-85d05e1e275d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-30 | |||||
| タイトル | ||||||
| タイトル | What we know about the communication between carbon-irradiated cancer cells and bystander human fibroblasts | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapromporn, Narongchai
× Autsavapromporn, Narongchai× Suzuki, Masao× Hua, Liu Cui× Murakami, Takeshi× アッサワプロンポーン ナロンチャイ× 鈴木 雅雄× 劉 翠華× 村上 健 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the role of intercellular communication and oxidative metabolism in determining the biological responses of human cells to heavy ion radiotherapy. We focused on the communication of signaling events between carbon-irradiated cancer cells and bystander normal cells. Here, human glioblastoma cells (T98G) were irradiated with a single dose of 6 Gy or doses divided into 3 fractions given at consecutive days (3 x 2 Gy) of high-LET carbon ions (LET -76 keV/um). Then were co-cultured with normal human skin fibroblasts (NB1RGB), in the presence or absence of gap-junction inhibitor (18-alpha-glycyrrhetinic: AGA) or antioxidant (Superoxide Dismutase: SOD) for 4 hrs. The layered tissue culture system is used to allow co-culturing the irradiated-T98G cells growing in wells with bystander NB1RGB cells growing in inserts, and also medium transfer strategy. Bystander NB1RGB cells were then harvested and assayed for clonogenic survival, micronucleus formation (MN) or allowed to grow for 10 weeks and assayed for MN. As expected, relative to control, both single dose irradiation and fractionation of the dose into three fractions show the bystander NB1RGB cells exhibited reduced cloning efficiency and consistence with the increase of MN formation. In contrast, treatment with AGA or SOD shows a significant increase of survival and decrease of MN formation in bystander cells. Interestingly, the condition medium harvested from irradiated T98 cells receiving fractionated irradiation presented lower cloning efficiency and a higher MN formation than a single-dose irradiation. These results suggested that fractionated irradiation removed the effect of dose sparing that was observed after conventional fractionated regime. Furthermore, the progeny of bystander cells that were co-cultured with irradiated T98G cells for 10 weeks showed that there was induction of MN. Interestingly, the level of MN is reduced if there is the inhibition of GJIC. In addition, the findings also indicated that intercellular communication and oxidative metabolism amplifies the stressful effects of high-LET carbon ions in bystander cells and their progeny. Our results provide new evidence that high-LET carbon ions-induced the bystander effect and genomic instability in both single dose irradiation and fractionated irradiation. They were observed in bystander normal skin fibroblasts and their progeny indicating that irradiated cancer cells can induce damage in normal human cells. Characterizing the nature of the communicated molecules(s) would have translational implication in radiotherapy and radioprotection. Supported by the Japan Society for the Promotion of Sciences and the National Institute of Radiological Sciences |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 59th Radiation Research Society Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2013-09-19 | |||||
| 日付タイプ | Issued | |||||
