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A simple method for dehydrogenase assay of soil microorganisms to evaluate the biospheric behavior of C-14 originated in transuranic waste
https://repo.qst.go.jp/records/71264
https://repo.qst.go.jp/records/71264038cce61-332a-4118-b69e-488c3771b010
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-30 | |||||
| タイトル | ||||||
| タイトル | A simple method for dehydrogenase assay of soil microorganisms to evaluate the biospheric behavior of C-14 originated in transuranic waste | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Iwata, Kayoko
× Iwata, Kayoko× Ishii, Nobuyoshi× Tagami, Keiko× Uchida, Shigeo× 岩田 佳代子× 石井 伸昌× 田上 恵子× 内田 滋夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | When carbon-14 (14C) that originated in transuranic (TRU) waste reaches the ground surface in low molecular weight organic forms, it is reported that microbial activity has influence on 14CO2 gas production. This study aims to develop a simple method to determine soil microbial respiratory activity, which results can be applied to evaluate their correlations with 14C gasification ratios. Respiratory activity was measured using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetraxolium chloride (INT), which is reduced to INT-formazan by the dehydrogenase enzyme. Soil samples were incubated at a solid: liquid ratio of 1 to 10, often used in the gasification ratio experiments. Our experiment showed that three-hour incubation with INT was appropriate. Vortexing samples with 4mL of methanol for one minute sufficiently extracted the resultant INT-formazan. Extracted INT-formazan was stable in the dark at both 4oC and room temperature. These procedures and following results, considering the gasification ratio experiments, simply enable to examine the relationship between microbial activity and 14CO2 gasification ratio. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The fifth international symposium in the series of Asia-Pacific Symposium on Radiochemistry | |||||
| 発表年月日 | ||||||
| 日付 | 2013-09-27 | |||||
| 日付タイプ | Issued | |||||
