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Intercellular Communication Between Carbon-Irradiated Cancer Cells and Non-Irradiated Bystander Fibroblasts: The Impact of Fractionated Irradiation
https://repo.qst.go.jp/records/71151
https://repo.qst.go.jp/records/711519fe3a56a-e73c-4bea-b6b7-7a0b54928e95
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-17 | |||||
| タイトル | ||||||
| タイトル | Intercellular Communication Between Carbon-Irradiated Cancer Cells and Non-Irradiated Bystander Fibroblasts: The Impact of Fractionated Irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapromporn, Narongchai
× Autsavapromporn, Narongchai× Suzuki, Masao× Hua, Liu Cui× Murakami, Takeshi× アッサワプロンポーン ナロンチャイ× 鈴木 雅雄× 劉 翠華× 村上 健 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Aim: Radiation-induced bystander effect (RIBE) has important implication in radiotherapy (1-3), but the bystander signal between irradiated cancer cells and bystander cells are still not well known. Our experiments were aimed at evaluating this concept. Method: Human glioma (T98G) cells were irradiated with a single doses of 6 Gy or doses divided into 3 fractions given at consecutive days (3 x 2 Gy) of carbon ions and then co-cultured with fibroblasts (NB1RGB), in the presence or absence of gap-junction inhibitor AGA for 4 h using the layered tissue culture system which allows co-culturing the irradiated-T98G cells growing in wells with bystander NB1RGB cells growing in inserts (2), and medium transfer strategy (3). Bystander NB1RGB cells were then harvested and assayed for clonogenic survival and micronucleus formation (MN). Results: Relative to control, both single dose irradiation and fractionation of the dose into three fractions show the bystander NB1RGB cells exhibited reduced cloning efficiency and consistence with increased of MN formation. In contrast, treatment with AGA shows a significant increase of survival and decrease of MN formation in bystander cells. These indicated that gap-junction intercellular communication in the propagation of RIBE. Interestingly, the condition medium harvested from irradiated T98 cells receiving fractionated irradiation presented lower cloning efficiency and a higher MN formation than a single-dose irradiation. These results suggested that fractionated irradiation removed the effect of dose sparing that is observed after conventional fractionated regime. Conclusions: Our results provide the evidence that RIBE in both single dose irradiation and fractionated irradiation, which were observed in bystander fibroblasts indicating that irradiated cancer cells can induce damage in normal cells. Characterizing the nature of the communicated molecules would have translational implications in radiotherapy and radioprotection. References 1. Autsavapromporn N., Suzuki M., Funayama T., Usami U., Plante I., Yokota Y., Mutou Y., Ikeda H., Uchihori Y., Kobayashi K., Kobayashi Y., Hei T.K., Azzam E.I. and Murakami T. Gap Junction Communication in the Propagation of Bystander Effect in Human Fibroblasts induced by Microbeams: The Impact of Radiation Quality, Radiation Research (Submitted) 2. Wakatsuki M., Magpayo N., Kawamura H. and Held K.D. Differential Bystander Signaling Between Radioresistant Chondrosarcoma Cells and Fibroblasts After X-ray, Proton, Iron Ion and Carbon Ion Exposures. International Journal of Radiation Oncology, Biology, Physics (2012), 84: e103-e108. 3. Mothersill C. and Seymour C.B. Bystander and Delayed Effects after Fractionated Radiation Exposure. Radiation Research (2002), 158: 626-633. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 52nd Annual Conference of the Particle Therapy Co-Operative Group | |||||
| 発表年月日 | ||||||
| 日付 | 2013-06-08 | |||||
| 日付タイプ | Issued | |||||
