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Diffusion mapping to quantify tumor initiation and prevention in mouse radiation lymphomagenesis
https://repo.qst.go.jp/records/71129
https://repo.qst.go.jp/records/711299e4e669a-6882-4a73-8e33-182a79c500ba
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-03 | |||||
| タイトル | ||||||
| タイトル | Diffusion mapping to quantify tumor initiation and prevention in mouse radiation lymphomagenesis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hasegawa, Sumitaka
× Hasegawa, Sumitaka× Aoki, Ichio× Furukawa, Takako× Saga, Tsuneo× 長谷川 純崇× 青木 伊知男× 古川 高子× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction Quantitative analysis of cellular events during the tumorigenic process represents a major challenge in the field of molecular imaging of cancer. In mouse radiation-induced thymic lymphoma (RITL), events of post-irradiation in the bone marrow (BM) such as damage and regeneration are critical as a tumor initiation for RITL development. However, the in vivo cellular dynamics remains elusive due to a lack of a direct and quantitative measurement. We investigated if diffusion-weighted imaging (DWI) and the measurement of apparent diffusion coefficient (ADC) are useful for quantitatively evaluating BM changes of post-irradiation. Methods Four-week-old C57BL/6 mice were applied to fractionated whole-body X-irradiation (FX) with 1.6 Gy/week for 4 consecutive weeks. In some irradiated mice, BM transplantation (BMT) was conducted by intravenously injecting more than 2x107 BM cells isolated from syngeneic GFP transgenic mice immediately (iBMT) or 4 weeks (dBMT) after FX. DWIs were acquired at two b-values (b = 1.62, 670 s/mm2) using 7T MRI with a sagittal single-slice SE echo planar imaging sequence. ADC maps were calculated from DWIs. All animal experiments were approved by the Institutional Animal Care and Use Committee. Results In unirradiated mice, BM ADCs has barely changed during the experiments (approximately 0.5 x 10-3/mm2/s). In contrast, BM ADC was increased from the first irradiation of FX, reaching the peak at second irradiation, thereafter decreasing until 3 wk after FX in the irradiated mice. The value was restored to normal levels at 6-7 wk after FX. These changes were accounted for by pathological findings such as reduced cellularity, fatty changes, and regeneration in the BM. Interestingly, when treated FX-irradiated mice with iBMT, BM ADC was rapidly restored to normal level earlier than spontaneous recovery, whereas no accelerated recovery of BM ADC was found in the mice treated with dBMT. Given that iBMT, but not dBMT, prevents the onset of RITL, accelerated BM regeneration by iBMT is likely to be important for RITL prevention. Conclusions ADC maps can quantify BM damage and regeneration critical for RITL development, including the effect of BMT on the prevention. DWI measurement for ADC mapping would contribute to the better understanding of RITL development and provides a rationale for exploring the prevention for RITL-related human diseases. Acknowledgement / References This work was done by research fund of the NIRS, Japan. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | European Molecular Imaging Meeting - EMIM 2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-28 | |||||
| 日付タイプ | Issued | |||||
