WEKO3
アイテム
Response of 5 hpf zebrafish embryos to low-dose microbeam protons
https://repo.qst.go.jp/records/71110
https://repo.qst.go.jp/records/711106c052a72-5350-4cfe-8e0e-88c7cb64ffd0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-22 | |||||
| タイトル | ||||||
| タイトル | Response of 5 hpf zebrafish embryos to low-dose microbeam protons | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Choi, V.W.Y.
× Choi, V.W.Y.× Kobayashi, Alisa× Konishi, Teruaki× Oikawa, Masakazu× H., Cheng Shuk× Yu, K.N.× 小林 亜利紗× 小西 輝昭× 及川 将一 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to irradiate dechorionated embryos of the zebrafish, Danio rerio, at 5 h post fertilization (hpf) by microbeam protons each having an energy of about 3.4 MeV. In the first part of the experiments, only 1 position on the cells was irradiated, with 10, 20, 40, 80, 100, 200, 400, 500, 600, 800, 1000 or 2000 protons. In the second part of the experiments, 10 separate positions on the cells were irradiated, each with 5, 10, 20, 40, 50, 60, 80, 100, 200, 500, 800 or 1000 protons. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay, with the apoptotic signals captured by a confocal microscope. Both parts of experiments revealed a triphasic dose-response for zebrafish embryos, including (1) a sub-hormetic zone with an increase in apoptotic signals for a small number of irradiated protons per position, (2) a hormetic zone with a reduction of the apoptotic signals below the spontaneous level for a larger number of irradiated protons per position, and (3) a toxic zone with an increase in apoptotic signals again if the number of irradiated protons per position was further increased. When comparing the two experiments, it was interesting to note that the transition from the sub-hormetic zone to the hormetic zone occurred for a similar number of irradiated protons per position, while the transition from the hormetic zone to the toxic zone occurred for a similar number of total irradiated protons. Possible reasons would be discussed. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Heavy Ion in Therapy and Space Radiation Spymposium 2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-18 | |||||
| 日付タイプ | Issued | |||||
