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Roles of nitric oxide in adaptive response induced in zebrafish embryos in vivo by microbeam protons
https://repo.qst.go.jp/records/71108
https://repo.qst.go.jp/records/711088eb57328-4af6-4f42-8a7b-aea511f2f02c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-22 | |||||
| タイトル | ||||||
| タイトル | Roles of nitric oxide in adaptive response induced in zebrafish embryos in vivo by microbeam protons | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Choi, V.W.Y.
× Choi, V.W.Y.× Y.P., Ng Candy× Kobayashi, Alisa× Konishi, Teruaki× Oikawa, Masakazu× H., Cheng Shuk× Yu, K.N.× 小林 亜利紗× 小西 輝昭× 及川 将一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radioadaptive response (RAR) was successfully induced in dechorionated (5 hpf) embryos of the zebrafish, Danio rerio, by 3.4 MeV protons from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), against a challenging exposure of 2 Gy of X-ray irradiation at 10 hpf. The RAR induction was evidenced by reduced apoptotic signals at 25 hpf revealed through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay. If de novo synthesis of factors was required for RAR induction, these should have already been synthesized at 5 h after the priming dose. Application of a nitric oxide scavenger 2-(4-Carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) to the medium at 0, 1, 2, 3 or 5 h after application of priming exposure significantly suppressed RAR. The suppression of RAR with the application of cPTIO to the medium at 5 h after the priming dose irradiation, where de novo synthesis of factors should have been completed, suggested that NO scavenging impaired the repair machineries in the bystander cells. The suppression of RAR with the application of cPTIO to the medium at earlier than 5 h after the priming dose irradiation could be explained by the scavenging of bystander NO signals in the medium and thus deterring the de novo synthesis of factors. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Heavy Ion in Therapy and Space Radiation Spymposium 2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-18 | |||||
| 日付タイプ | Issued | |||||
