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Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity
https://repo.qst.go.jp/records/71062
https://repo.qst.go.jp/records/71062397dc62b-a348-4f34-9377-e5397377b559
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-03-08 | |||||
| タイトル | ||||||
| タイトル | Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Mayumi
× Fujita, Mayumi× Imadome, Kaori× Shoji, Yoshimi× Imai, Takashi× 藤田 真由美× 今留 香織× 荘司 好美× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have previously reported that Carbon ion (C-ion) irradiation suppresses the invasiveness of several pancreatic cancer cell lines, MIAPaCa-2, BxPC-3, and AsPC-1 cells, however, we also observed that C-ion irradiation enhanced invasion in PANC-1 cells, which occurred via the activation of serine proteases (SerP), plasmin, and uPA. Treatment of C-ion irradiated PANC-1 with SerP inhibitor and ROCK inhibitor resulted in reduced invasiveness, but only by 50%, suggesting that there are more factors that have a role in C-ion-irradiation-induced PANC-1 invasion. To study the molecular factors involved in irradiation-induced PANC-1 invasion, we used the Screening Committee of Anticancer Drugs (SCADS) compound library and found that nitric oxide synthase (NOS) inhibitors and phosphatidylinositol 3-kinases (PI3K) inhibitors were both effective in reducing PANC-1 invasion. Most invaded PANC-1 cells were nitric oxide (NO)-producing cells, and the NOS-PI3K-AKT pathway was activated in these cells. This effect could be abrogated by NOS inhibitor treatment, which also reduced invasion. The population of NO-producing cells was increased by C-ion irradiation, thereby enhancing C-ion-irradiation-induced PANC-1 invasion. These results suggested that irradiation alters the invasive potential through NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ninth AACR-Japanese Cancer Association Joint Conference | |||||
| 発表年月日 | ||||||
| 日付 | 2013-02-25 | |||||
| 日付タイプ | Issued | |||||
