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Profiling Genetic Changes at Common Tumor Suppressor Loci in Carbon Ion Radiation-Induced Thymic Lymphomas
https://repo.qst.go.jp/records/71051
https://repo.qst.go.jp/records/710517d8474fc-0e9e-4693-bbfb-58e008185837
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-02-26 | |||||
| タイトル | ||||||
| タイトル | Profiling Genetic Changes at Common Tumor Suppressor Loci in Carbon Ion Radiation-Induced Thymic Lymphomas | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Blyth, Benjamin
× Blyth, Benjamin× Kakinuma, Shizuko× Amasaki, Yoshiko× Shang, Yi× Sawai, Tomoko× Hirano, Shinobu× Tsuruoka, Chizuru× Nishimura, Mayumi× Shimada, Yoshiya× Ｂｌｙｔｈ Ｂｅｎｊａｍｉｎ× 柿沼 志津子× 甘崎 佳子× 尚 奕× 澤井 知子× 坂入 しのぶ× 鶴岡 千鶴× 西村 まゆみ× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Secondary cancer risk following carbon ion radiotherapy is a key consideration for its use in children over conventional photon radiotherapy. Understanding not only the relative carcinogenic risk of carbon ion therapy, but also the potentially different carcinogenic mechanisms is a primary goal of the Radiobiology for Childrens Health Research Program at the National Institute of Radiological Sciences, Japan. The research program has undertaken a large-scale study over the past 8 years, for which cohorts of irradiated B6C3F1 mice are followed throughout their natural life for tumour development. The study includes groups which vary in their age-at-exposure, as well as radiation source, quality, fractionation and total dose. From this study, a sub-cohort of mice was selected for detailed genetic analysis of radiation-induced thymic lymphomas. This included all mice irradiated with 4 - 4.8 Gy carbon ions starting at one week of age (either in a single exposure, or four weekly fractions), which had been sacrificed in a moribund state with a cause of death at autopsy of thymic lymphoma (n 102 mice). DNA and RNA were isolated from the corresponding thymic lymphoma tissue bank samples. Gross genomic changes at tumour suppressor loci including Pten, Bcl11b and Ikzf1(IKAROS family zinc finger 1) are being assessed by loss of heterozygosity (LOH) analysis using PCR, and amplicon sizing of sites polymorphic for sequence length between the B6 and C3H parental strains. Smaller genetic alterations are being further assessed by exon sequencing of Pten, Bcl11b and Ikzf1 to identify small insertions/deletions and point-mutations. The features observed in the carbon ion-irradiated sub-cohort will be compared to those in a reference population of tumours in mice irradiated with a corresponding regime of gamma-radiation. Preliminary results suggest universal LOH in all carbon ion radiation-induced thymic lymphomas at sites flanking Bcl11b (compared to only 69pc in gamma radiation-induced thymic lymphomas), and approximately twice the frequency of LOH at a site -4 kb downstream of Pten (90pc vs. 43pc). Mechanistic insight into carbon ion-induced carcinogenesis will be vital in assessing the long-term safety of carbon ion radiotherapy for children. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 平成24年度「個体レベルでのがん研究支援活動」ワークショップ 個体レベルのがん研究による相乗効果 学術的インターラクションから創造へ | |||||
| 発表年月日 | ||||||
| 日付 | 2013-02-07 | |||||
| 日付タイプ | Issued | |||||
