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Analysis in expression of CD180 molecules on spleen cells after radiation-induced apoptosis in vivo
https://repo.qst.go.jp/records/71007
https://repo.qst.go.jp/records/71007ac6d4988-94cb-46cf-95b8-50ea71a168de
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-11 | |||||
| タイトル | ||||||
| タイトル | Analysis in expression of CD180 molecules on spleen cells after radiation-induced apoptosis in vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Kazuko
× Fujita, Kazuko× Kuwabara, Taku× Bing, Wang× Tanaka, Kaoru× Akasaka, Yosikiyo× Ishii, Toshiharu× 藤田 和子× 桑原 卓× 王 冰× 田中 薫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The CD180 molecule is a receptor of LPS on mature B cell and a homologue of TLR 4, and regulates TLR 4 negatively. The molecule has a protective effect against radiation- or dexamethasone-induced apoptosis in vitro. Antibody-mediated cross-linkingof CD180 induces a strong proliferative response, and secondary cross-linking of surface IgM induces B cell apoptosis in vitro. CD180 is thus a key regulator of proliferation and cell death. However, few studies regarding the functional mechanism of the CD180 molecule associated with radiation-induced apoptosis have been reported in vivo. In the present study, we have examined the effect of irradiation on the expression patterns of CD180 of spleen cell and compared with that of CD19 and CD21 on B cell in vivo. Spleens of BALB/c female mice at 7-8 weeks were removed after whole body-irradiation of 4 Gy. Histochemical analysis demonstrated that CD180-positive lymphocytes significantly increased in number at 6 h after irradiation compared with both at 3 h and 0 h. In contrast, no significant change of the numbers of lymphocytes positive for CD19 or CD22 was at 0 h, 3 h, and 6 h after irradiation. Flow cytometric analysis showed that the percentage of CD180-presenting B cells increased at 6 h after irradiation than that at 0 h. As the expression of CD180 was increased dependently on radiation dose from 0 Gy to 10 Gy and on the level of apoptosis with histochemical analysis, double staining for TUNEL and CD180 was performed to clarify the relationship of the expression patterns between apoptosis and CD180. The double staining showed that the number of CD180-positive cells positive for TUNEL was significantly less than that of CD180-negative cells positive for TUNEL at 6 h after irradiation. These results suggested that CD180- positive B lymphocytes were resistant to apoptosis induced by irradiation in vivo. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第41回日本免疫学会学術集会 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-12-07 | |||||
| 日付タイプ | Issued | |||||
