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Evaluation of cell survival and micronucleus formation in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays
https://repo.qst.go.jp/records/70947
https://repo.qst.go.jp/records/7094708739a8e-0216-47fd-9a93-e1436780c54a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-10-26 | |||||
| タイトル | ||||||
| タイトル | Evaluation of cell survival and micronucleus formation in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirayama, Ryoichi
× Hirayama, Ryoichi× Uzawa, Akiko× Takase, Nobuhiro× Koda, Kana× Matsumoto, Yoshitaka× Ozaki, Masakuni× Noguchi, Miho× Kase, Yuki× Matsufuji, Naruhiro× Koike, Sachiko× Masunaga, Shinichiro× Ando, Koichi× Okayasu, Ryuichi× Furusawa, Yoshiya× 平山 亮一× 鵜澤 玲子× 幸田 華奈× 松本 孔貴× 尾崎 匡邦× 松藤 成弘× 小池 幸子× 岡安 隆一× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study was to clarify the RBE (relative biological effectiveness) and OER (oxygen enhancement ratio) for cell killing and micronucleus formation in clamped and non-clamped transplanted solid tumors in mice leg after 290 MeV/n carbon-ion beams and X-rays. Furthermore, single-cell suspensions were prepared by tumors and were used directly to determine the radiation response of oxic cells. Squamous cell carcinoma (SCC VII) cells were transplanted into the right hind legs of syngeneic C3H male mice. Irradiation with either carbon-ion beams with a 6-cm spread-out Bragg peak (46 and 80 keV/µm) or X-rays was delivered to 5-mm diameter tumors. Single-cell suspensions were prepared from excised and trypsinized tumors, and were used for in vivo-in vitro colony formation assay to obtain cell-survival curves. Moreover, a large portion of single-cell suspensions were incubated with cytochalasin-B to examine the dose-response curves for micronucleus (MN) formation. The RBE and OER for cell survival were evaluated by D10 values (the dose required to reduce surviving fraction down to 10%), and the RBE and OER for MN formation were evaluated by D0 values (the dose corresponding to 37% retained binucleated cell without micronuclei. The RBEs for survival and MN formation increased with increasing LET. These maximum values were 1.7-2.4 at an LET of 80 keV/µm. The OERs for in vivo (clamped tumor / non-clamped tumor) were smaller than that for in vitro (clamped tumor / single-cell suspension) in both endpoints, and the dependence of OERs for in vivo toward LET was small in comparison to the OERs for in vitro. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 10th International Symposium on Chromosomal Aberrations | |||||
| 発表年月日 | ||||||
| 日付 | 2012-10-21 | |||||
| 日付タイプ | Issued | |||||
