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Intratumoral distribution of PET molecular probes in CTOS xenograft, a tumor model retaining the properties of the original tumor.
https://repo.qst.go.jp/records/70890
https://repo.qst.go.jp/records/7089033722599-51ed-4baf-bd91-3907730e72ff
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-09-12 | |||||
| タイトル | ||||||
| タイトル | Intratumoral distribution of PET molecular probes in CTOS xenograft, a tumor model retaining the properties of the original tumor. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Furukawa, Takako
× Furukawa, Takako× Yoshii, Yukie× Fujibayashi, Yasuhisa× Saga, Tsuneo× et.al× 古川 高子× 吉井 幸恵× 藤林 康久× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Cancer cell lines and their xenografts are useful models to study cancer biology and to evaluate diagnostic or therapeutic drugs. Although they have played essential roles in the research, in some cases, their loss of properties of the original tumor could make it difficult to interpret the experimental results. For example, we have experienced different intratumoral distribution patterns between FDG and Cu-ATSM in xenograts of various cancer cell lines, although the intratumoral distribution of the two PET probes were reported to show positive correlation in adenocarcinomas in clinical studies (1,2). Cancer tissue originated spheroid (CTOS) is a recently developed, unique culture method that enables to preserve the properties of the original tumor (3). We examined the intratumoral distribution of FDG and Cu-ATSM in CTOS xenografts to see if the distribution reflects the clinical findings. Methods: The CTOSs derived from colon adenocarcinoma were subcutaneously transplanted into immunodeficient mice. When tumors of 10-20 mm in diameter were formed, double tracer autoradiography of [14C]FDG and [64Cu]Cu-ATSM and histological staining were performed on the xenografts and compared with that of the xenografts formed by transplantation of HT29, a cancer cell line derived from a colon adenocarcinoma. Results and discussion: CTOS xenografts showed hitological characteristics of adenocarcinoma, while HT29 xenografts lacked the characteristics. In HT29 xenografts, FDG accumulated in the area toward the center of the tumor, closer to the necrotic area, compared with Cu-ATSM, which accumulated in the area toward the periphery, surrounding the area with high accumulation of FDG. In contrast, the accumulations of FDG and Cu-ATSM in CTOS xenografts were overlapped in many areas, although there were areas with mismatched distributions. Comparison of the autoradiography with the histological staining indicated that the area of high accumulation of the two PET probes could have different properties but the rather complicated, glandular-like structure of the CTOS xenograft, which resembled the original tumor, brought the overlapped distribution. CTOS xenogrft would provide an excellent tumor model for molecular imaging research, both for translational and reverse-translational studies. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2012 World Molecular Imaging Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2012-09-08 | |||||
| 日付タイプ | Issued | |||||
