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Genome-wide changes of radiation-induced mammary carcinoma of (Sprague-Dawley & Copenhagen) F1 hybrid rats overlapping with those of human breast cancers
https://repo.qst.go.jp/records/70826
https://repo.qst.go.jp/records/70826a6227671-f904-47da-b48b-10a3dd2b580d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-07-18 | |||||
| タイトル | ||||||
| タイトル | Genome-wide changes of radiation-induced mammary carcinoma of (Sprague-Dawley & Copenhagen) F1 hybrid rats overlapping with those of human breast cancers | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nishimura, Mayumi
× Nishimura, Mayumi× Daino, Kazuhiro× Takabatake, Takashi× Fukuda, Maki× Tanaka, Ikuya× Takabatake, Masaru× Imaoka, Tatsuhiko× Kakinuma, Shizuko× Shimada, Yoshiya× 西村 まゆみ× 臺野 和広× 高畠 貴志× 福田 真希× 田中 郁也× 高畠 賢× 今岡 達彦× 柿沼 志津子× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: The breast tissue is one of the most susceptible organs to radiation-induced carcinogenesis. But, most studies, however, have failed in identifying clear genetic changes in radiation-induced breast/mammary cancers. In the present study, we examined the candidate genes in the radiation-induced rat mammary cancers, which exhibited genome-wide DNA copy number changes overlapping in part with those of human breast cancers. Material and Methods: F1 hybrid rats between mammary cancer susceptible Sprague-Dawley and resistant Copenhagen rats were exposed to 4 Gy gamma-rays at 7 weeks of age and underwent autopsy at the time of spontaneous death. Genome-wide DNA copy number was analyzed by array comparative genomic hybridization. Loss of heterozygosity (LOH), expression and methylation of candidate genes were also examined. Results: Copy number loss was more frequent than copy number gain. Copy number losses were identified in small regions of chromosomes 1q52, 2q12-15, 3q31-36 and whole chromosome 5. These included the syntenic regions of human chromosomes, 10q23, 5q11.2, 11p13-14, 1p36 and 8q21-22, where breast cancer susceptible loci and genomic deletions in the breast cancers are frequently reported. The lost region of chromosomes 1-3 and 5 contained breast cancer-related genes such as Pten, Pik3r1, Fas, Map3k1, Ercc8, Plk2, Il6st, Rad51, Bcl2l11 and Tp53bp1. Among these genes, expression of Fas, Ercc8 and Il6st was significantly reduced compared to those in the normal mammary gland. The loss in chromosomes 1 and 2 was either paternal or maternal with slight preference of parental origin. Conclusions: The loss of above chromosomal regions may be causative in radiation induction of rat mammary carcinoma through dysregulation of apoptosis and interleukin 6 pathways. This rat mammary cancer model would be informative for the elucidation of the mechanism of human breast carcinogenesis. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第２２回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-07-10 | |||||
| 日付タイプ | Issued | |||||
