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In-Vivo Tracking Of Transplanted Peripheral Mononuclear Cells Labeled With Manganese Using Magnetic Resonance Imaging
https://repo.qst.go.jp/records/70813
https://repo.qst.go.jp/records/70813e57857f8-3bce-40a5-8974-242e3955ec31
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-06-29 | |||||
| タイトル | ||||||
| タイトル | In-Vivo Tracking Of Transplanted Peripheral Mononuclear Cells Labeled With Manganese Using Magnetic Resonance Imaging | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Odaka, Kenichi
× Odaka, Kenichi× Aoki, Ichio× Ito, Koichi× Moriya, Junji× Tateno, Kaoru× Minamino, Tohru× Tadokoro, Hiroyuki× Shimoyama, Ichiro× Komuro, Issei× Saga, Tsuneo× Fukumura, Toshimitsu× 小高 謙一× 青木 伊知男× 森谷 純治× 舘野 馨× 田所 裕之× 佐賀 恒夫× 福村 利光 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Non-invasive high resolution imaging is required to track the cells and evaluate clinical relevance after cell transplantation. Manganese (Mn2+) is known to be an essential element for living organisms and there has been a recent renewed interest in Mn2+ as a useful contrast agent for magnetic resonance imaging (MRI). In-vivo tracking of transplanted peripheral mononuclear-cells (MNCs) with MRI has attempted to visualize the long term regenerative therapeutic effects and the localization of migrated cells at high spatial resolution in living tissues such as skeletal muscle, heart, and brain. We successfully established a method to label MNCs with Mn2+ (Mn-MNCs) and performed longitudinal observation of Mn-MNCs in a rat model of ischemic leg or myocardial infarction with 7-tesla MRI. Mn-MNCs or saline were intramuscularly injected into a border zone of the ischemic lesion and were evaluated by T1-weighted imaging, repeatedly. The transplanted Mn-labeled MNCs were typically detected as a "double-layered" structure having a negative core with surrounding positive enhancement immediately after the intramuscular administration, and those were clearly observed for up to 21 days in T1-weighted MRI. This Mn-enhanced method enables visualization of the transplanted area at 150-175 µm in-plane spatial resolution and allows the migration of labeled-MNCs to be observed for long periods in the same animal. In addition, manganese deposition was not detected when Mn-labeled mononuclear cells were transplanted into brain striatum. Mn2+-enhanced cellular MRI promises to be a useful technique for the quantitative evaluation of cell tracking. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第10回国際幹細胞学会総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-06-16 | |||||
| 日付タイプ | Issued | |||||
