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Effects of Carbon Ion Beams on Liver Cancer Stem-Like Cells and Its Comparison with X-rays
https://repo.qst.go.jp/records/70808
https://repo.qst.go.jp/records/70808348f74a0-1a80-4edc-9420-177b2484ae8b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-06-22 | |||||
| タイトル | ||||||
| タイトル | Effects of Carbon Ion Beams on Liver Cancer Stem-Like Cells and Its Comparison with X-rays | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Oonishi, Kazuhiko× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 大西 和彦× 山田 滋× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate whether a carbon ion beam has beneficial effects compared to X-rays by targeting putative cancer stem cells, human liver cancer stem-like cells sorted from HepG2 and Huh7 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, in vivo tumorigenesis analyses, FACS analysis, as well as gammaH2AX foci formation assay were performed. FACS analysis showed that the proportion of CD133+/CD90+, CD44+/ESA+ was more significantly enriched by X-rays compared to carbon-ion beam. CD133+/CD90+ and CD44+/ESA+ cells significantly have higher number of colony and spheroid compared to CD133-/CD90- and CD44-/ESA- cells. In addition, CD133+/CD90+ and CD44+/ESA+ cells have shown more potential to form tumors in SCID mice than CD133-/CD90- and CD44-/ESA- cells. Colony assay showed that CD133+/CD90+ and CD44+/ESA+ cells appeared to be radioresistant to both X-ray and carbon ion beam, but carbon beam was more effective at killing those of cancer stem-like cells. The number of gammaH2AX foci in CD44-/CD24- cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of gammaH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists for significantly longer in carbon ion beam irradiated cells compared to X-rays. In conclusion, CD133+/CD90+ and CD44+/ESA+ cells are putative liver cancer stem cells, and carbon ion beams induce prolonged DNA damage, therefore potentially have advantage by targeting liver cancer stem-like cells compared to X-rays. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Society for Stem Cell Research (ISSCR) 10 th Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2012-06-16 | |||||
| 日付タイプ | Issued | |||||
