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Alteration in the DNA methylation profile of irradiated cancer cell lines
https://repo.qst.go.jp/records/70704
https://repo.qst.go.jp/records/7070400b7674f-2c0b-42ce-8e58-9d8838552394
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-02-24 | |||||
| タイトル | ||||||
| タイトル | Alteration in the DNA methylation profile of irradiated cancer cell lines | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishikawa, Kenichi
× Ishikawa, Kenichi× Ishikawa, Atsuko× Shoji, Yoshimi× Kanai, Akinori× Iizuka, Daisuke× Imai, Takashi× 石川 顕一× 石川 敦子× 荘司 好美× 飯塚 大輔× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA methylation is one of the epigenetic modifications that occurs in genome of mammals to ensure the proper regulation of gene expression in response to environmental stimuli and stable gene silencing. However, the regulation of gene expression by DNA methylation after genotoxic stress including irradiation remains poorly understood. We hypothesized that irradiation may elicit alterations in the methylation status of DNA and subsequently alter gene expression. In this study, we have attempted to integrate the result of comprehensive gene expression analysis and DNA methylation analysis in cellular response to irradiation in MIAPaCa-2 and PANC-1 cell lines, both derived from human pancreatic cancer. Gene expression in these cell lines at 48 h after irradiation with X-ray at 4 Gy, or a carbon ion beam at 2 Gy were analyzed by microarray. DNA methylation status in the cell lines in these conditions were analyzed by next generation sequencing. Methylated DNA fragments were concentrated using methyl binding protein after fragmentation of DNA to an average fragment size of 200 bp. Genome regions showing a different methylation status after irradiation were detected using MACS algorithm. In total, 62 genes or 226 genes changed their expression by X-ray or carbon ion beam irradiation. 45 genes (73 %) of the 62 genes altered DNA methylation status while 186 genes (82 %) of 226 genes altered DNA methylation status by the irradiation. Expressions of some radio-responsive genes were also measured in the cells treated with 5-aza-2'-deoxycytidine reagent. Expressions of these radio-responsive genes were cell line specific, whereas expression of 38 genes responded to both X-ray and carbon ion beam irradiation. Studies are in progress to examine the relationship between alteration of DNA methylation status and gene expression in cells following irradiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Symposium for 50th Anniversary of RIRBM, Hiroshima University | |||||
| 発表年月日 | ||||||
| 日付 | 2012-02-21 | |||||
| 日付タイプ | Issued | |||||
