WEKO3
アイテム
Mouse Spontaneous Liver Metastasis Model from Orthotopic Human Colon Cancer Xenograft Stably Expressing Human Sodium-Iodide Symporter Reporter Gene
https://repo.qst.go.jp/records/70540
https://repo.qst.go.jp/records/705400449bc35-5689-464f-9056-ecfb321ee422
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-20 | |||||
| タイトル | ||||||
| タイトル | Mouse Spontaneous Liver Metastasis Model from Orthotopic Human Colon Cancer Xenograft Stably Expressing Human Sodium-Iodide Symporter Reporter Gene | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Inubushi, Masayuki
× Inubushi, Masayuki× Yong-Nan, Jin× Murai, Chika× Hata, Hironobu× Takeuchi, Yasuto× Xu, Hua× Tsuji, Atsushi× Koizumi, Mitsuru× Kitagawa, Yoshimasa× Saga, Tsuneo× 犬伏 正幸× 村井 知佳× 竹内 康人× 徐 華× 辻 厚至× 小泉 満× 佐賀 恒夫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Subcutaneous human tumor xenografts in immunodeficient mice do not sufficiently represent clinical cancer especially with regard to metastasis. In this study, we therefore developed a mouse spontaneous liver metastasis model from an orthotopic human colon cancer xenograft stably expressing a human sodium-iodide symporter (NIS) reporter gene, of which metastasis can be tracked noninvasively and with 99mTcO4- SPECT or 124I PET. Methods: A recombinant plasmid containing NIS constitutively driven by a cytomegalovirus promoter (pcDNA3-NIS) was transfected into a human colon cancer cell line HCT116, and the stable cell lines were generated. Functional NIS expression in these cells was confirmed by 99mTcO4- uptake assay and Western blotting analysis in vitro. Then, suspensions of the stable cell lines were injected into the cecal walls of nude mice. When the mice developed abdominal masses, the tumors were excised, cut into small pieces, and implanted orthotopically into the cecal walls of different nude mice. Results: Six to eight weeks after the implantation, 99mTcO4- SPECT/CT imaging was performed using a dedicated small animal SPECT/CT system (Gamma Medica-Ideas, Northridge, CA), and focal 99mTcO4- uptakes were detected in the liver. Immunohistochemical analyses of the liver tumors demonstrated NIS expression in the cells, indicating metastases from the NIS expressing orthotopic colon xenografts. Conclusion: We developed a mouse spontaneous liver metastasis model from an orthotopic human colon cancer xenograft, and successfully imaged the metastatic tumors in vivo with 99mTcO4- SPECT. This unique animal model of clinically relevant and externally detectable liver metastases will be useful for studying the mechanism of cancer metastasis and the evaluation of novel anticancer drugs. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2011 World Molecular Imaging Congress (WMIC) | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-10 | |||||
| 日付タイプ | Issued | |||||
