WEKO3
アイテム
Loss of heterozygosity and copy number changes in the genome of radiation-induced mammary carcinoma of (Sprague-Dawley x Copenhagen) F1 hybrid rats
https://repo.qst.go.jp/records/70520
https://repo.qst.go.jp/records/70520f18307ed-1763-497b-ab54-f9827eb1a236
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-09 | |||||
| タイトル | ||||||
| タイトル | Loss of heterozygosity and copy number changes in the genome of radiation-induced mammary carcinoma of (Sprague-Dawley x Copenhagen) F1 hybrid rats | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nishimura, Mayumi
× Nishimura, Mayumi× Imaoka, Tatsuhiko× Takabatake, Takashi× Fukuda, Maki× Daino, Kazuhiro× Kakinuma, Shizuko× Takabatake, Masaru× Shimada, Yoshiya× 西村 まゆみ× 今岡 達彦× 高畠 貴志× 福田 真希× 臺野 和広× 柿沼 志津子× 高畠 賢× 島田 義也 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Epidemiological studies indicate that breast is one of the most susceptible organs to radiation-induced carcinogenesis. Most studies, however, have failed in identifying clear genetic alterations in radiation-induced breast/mammary cancers. We therefore aimed at identifying genomic changes in the radiation-induced rat mammary cancers. Experimental procedures: F1 hybrid rats were generated by crossing mammary cancer susceptible Sprague-Dawley females with resistant Copenhagen males. They were exposed to 4 Gy gamma-rays at 7 weeks of age and underwent autopsy at the time of spontaneous death. Genome-wide DNA copy number was analyzed by array comparative genomic hybridization. Loss of heterozygosity (LOH) and quantitative RT-PCR analyses were also performed. Results: Copy number losses were identified in small regions of chromosomes 1q52, 2q12-15 and 3q31-36. These correspond to the syntenic regions of human chromosomes, 10q23, 5q11.2 and 11p13-14, respectively, where genomic loci is frequently reported to be deleted. LOH was observed frequently in chromosomes 1 and 2; the loss was either paternal or maternal depending on the chromosome. The lost region of chromosomes 1-3 contained breast cancer-related genes such as Pten, Pik3r1, Fas, Map3k1, Wt1, Il6st, Rad51, Bcl2l11 and Tp53bp1. Among these genes, expression of Fas and Il6st was significantly lower than that in the normal mammary gland. Conclusions: The loss of above chromosomal regions may be causative in radiation induction of rat mammary carcinoma through abrogation of apoptosis and interleukin 6 pathways. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-01 | |||||
| 日付タイプ | Issued | |||||
