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Radiosensitivity of neural stem cells derived from mouse embryonic stem cells: Proliferation, cell cycle regulation and DNA double-strand break repair
https://repo.qst.go.jp/records/70506
https://repo.qst.go.jp/records/705069f192bc6-0295-4472-a2fe-b9dc54744f10
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-05 | |||||
| タイトル | ||||||
| タイトル | Radiosensitivity of neural stem cells derived from mouse embryonic stem cells: Proliferation, cell cycle regulation and DNA double-strand break repair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Isono, Mayu
× Isono, Mayu× Konishi, Teruaki× Shiomi, Naoko× Suya, Noriyoshi× Inoue, Nobuo× 小西 輝昭× 塩見 尚子× 酢屋 徳啓 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation exposure to developing fetal period is known to cause neuronal disorders, such as neuronal dysfunction, microcephaly and mental retardation, which are considered mainly due to the radiation damage produced in neural stem cells (NSCs). NSCs, which are the dominant number in the fetal brain, have an ability to proliferate itself, and differentiate into other cell types that construct the central nervous system (CNS), such as neurons, astrocytes and oligodendrocytes. Recently, it has been reported that NSCs exist in an adult brain. Thus, investigation on radiation biological effects against NSCs would be one of the important studies for radiation risk assessment, such as for the field of radiation therapy, and health risks against space environmental radiation exposure. The purpose of this study was to examine the cellular responses of proliferating NSCs against X-ray irradiation. Following biological endpoints were measured to demonstrate the radio-sensitivity of NSCs: maintenance of its ability to proliferate as NSCs, growth curve, cell cycle regulation and DNA repair. The NSCs were prepared from embryonic stem cells (HK cell line) of C57BL/6 mice using Neural Stem Sphere method that has established originally. The cells were irradiated up to 10 Gy by 200 kVp X-ray. Our findings demonstrated that NSCs, which survived after X-ray irradiation, maintained its ability as stem cells. Cellular responses, such as cell cycle arrest due to the DNA damage recognized at G1 and G2/M check points, and the linear-quadratic repair kinetics of DNA double-strand break repair, were similar to other mammalian cell lines. Further investigation on radiation sensitivities of NSCs in proliferation condition is necessary, and clarifying the correlation between the radiation-induced DNA damage in proliferative NSCs and its effect in the differentiation pathway will give the important information on radiation risks against in CNS. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-01 | |||||
| 日付タイプ | Issued | |||||
