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Induction and persistence of cytogenetic damage in cultured peripheral blood lymphocytes following 15 Gy gamma-irradiation

https://repo.qst.go.jp/records/70494
https://repo.qst.go.jp/records/70494
36bb60bb-32e2-47fc-ab4d-1c2519e7b23f
Item type 会議発表用資料 / Presentation(1)
公開日 2011-09-05
タイトル
タイトル Induction and persistence of cytogenetic damage in cultured peripheral blood lymphocytes following 15 Gy gamma-irradiation
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Suto, Yumiko

× Suto, Yumiko

WEKO 692241

Suto, Yumiko

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Akiyma, Miho

× Akiyma, Miho

WEKO 692242

Akiyma, Miho

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Hirai, Momoki

× Hirai, Momoki

WEKO 692243

Hirai, Momoki

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Yuuki, Masanori

× Yuuki, Masanori

WEKO 692244

Yuuki, Masanori

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Tominaga, Takako

× Tominaga, Takako

WEKO 692245

Tominaga, Takako

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Nakayama, Fumiaki

× Nakayama, Fumiaki

WEKO 692246

Nakayama, Fumiaki

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Suzuki, Tosikazu

× Suzuki, Tosikazu

WEKO 692247

Suzuki, Tosikazu

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Sugiura, Nobuyuki

× Sugiura, Nobuyuki

WEKO 692248

Sugiura, Nobuyuki

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Akashi, Makoto

× Akashi, Makoto

WEKO 692249

Akashi, Makoto

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數藤 由美子

× 數藤 由美子

WEKO 692250

en 數藤 由美子

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穐山 美穂

× 穐山 美穂

WEKO 692251

en 穐山 美穂

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平井 百樹

× 平井 百樹

WEKO 692252

en 平井 百樹

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結城 政則

× 結城 政則

WEKO 692253

en 結城 政則

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富永 隆子

× 富永 隆子

WEKO 692254

en 富永 隆子

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中山 文明

× 中山 文明

WEKO 692255

en 中山 文明

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鈴木 敏和

× 鈴木 敏和

WEKO 692256

en 鈴木 敏和

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杉浦 紳之

× 杉浦 紳之

WEKO 692257

en 杉浦 紳之

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明石 真言

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WEKO 692258

en 明石 真言

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抄録
内容記述タイプ Abstract
内容記述 In the conventional dicentric chromosome assay (DCA), the linear-quadratic dose response equation has been established for doses less than 6 Gy. At higher doses, DCA does not provide accurate dose response relationships due to mitotic delay, poor mitotic index and a high proportion of complex chromosomal aberrations. In case of partial body exposure, however, induction and transmission of chromosomal damage at high dose irradiation should be analyzed to assess the biological effects of radiation. The objective of this work is to characterize chromosomal aberrations induced by high-dose irradiation in cultured lymphocytes.
Peripheral blood samples were exposed to 15 Gy gamma-ray (60Co source, 0.51 Gy/min). Isolated lymphocytes were cultured in RPMI1640 medium supplemented with 20% fetal bovine serum, 2% phytohaemagglutinin and 30 mM bromodeoxyuridine (BrdU). Cells were harvested at 48 h, 50 h, 52 h, 54 h, 56 h, 72 h and 96 h after culture initiation. For the 72 h sample, multicolor fluorescence in situ hybridization (M-FISH) was conducted to analyze complex chromosome aberrations.
The first mitotic peak appeared at 52-54 h. Interestingly, cells containing aberrant chromosomes with as many as 8-10 centromeres were observed. Average dicentric equivalent count per cell ranged from 9.0 to 9.5 in 48 h – 56 h samples. In the 72 h sample, 20% of the dividing cells were in the 2nd metaphase. It should be noted that 70% of the 2nd metaphase cells were tetraploid cells including endoreduplicated cells. The high degree of polyploidy noted in our material suggests that irradiation may exert an effect on the replication process, in addition to the structural damage. M-FISH analyses revealed that in certain cells, aberrant chromosomes were accurately replicated in the polyploidization process. In the 96 h sample, cells at the third metaphase with octaploid chromosomes were found.
In conclusion, a certain population of peripheral blood lymphocytes was found to transit to the second and even third mitoses after high-dose in vitro irradiation, persisting with severe chromosome aberrations. The occurrence of polyploidization and endoreduplication following high-dose irradiation described here validated the reported fact that after high dose radiotherapy, tetrapliod cells were observed in the circulating blood of patients.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 14th International Comgress of Radiation Research
発表年月日
日付 2011-09-01
日付タイプ Issued
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