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Strain-dependent intra-alveolar hemorrhage with the accumulation of CD44 and Mac3 positive cells after carbon-ion irradiation
https://repo.qst.go.jp/records/70491
https://repo.qst.go.jp/records/704915fb56607-8032-4251-add3-94c68ef8209a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-05 | |||||
| タイトル | ||||||
| タイトル | Strain-dependent intra-alveolar hemorrhage with the accumulation of CD44 and Mac3 positive cells after carbon-ion irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Moritake, Takashi
× Moritake, Takashi× Fujita, Hidetoshi× Yanagisawa, Mitsuru× Nakawatari, Miyako× Imadome, Kaori× Nakamura, Etsuko× Iwakawa, Mayumi× Imai, Takashi× 盛武 敬× 藤田 英俊× 柳澤 充× 中渡 美也子× 今留 香織× 中村 悦子× 岩川 眞由美× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study was to investigate whether inherent factors produce variance of lung morbidity in response to carbon-ion (C-ion) irradiation, and to identify the molecules that have a key role in strain dependent lung adverse effects. Three strains of female mice (C3H/He Slc, C57BL/6J Jms Slc and A/J Jms Slc) were locally irradiated in the thorax with either C-ion beam (290 MeV/n, in 6 cm SOBP) or with 137Cs gamma-rays as a reference beam. We performed survival assays, histological examinations of the lung with hematoxylin-eosin staining, Masson s trichrome staining, immunohistochemical staining for CD44 and Mac3, and gene expression analyses. Intra-alveolar hemorrhage was assessed by Berlin blue staining and measurement of the iron concentration of bronchoalveolar lavage fluid. Survival data presented a mouse strain-variance after C-ion irradiation at 10 Gy. The histological examination revealed early phase hemorrhagic pneumonitis in C 3! H/He mice and late phase pulmonary fibrosis in C57BL/6J mice after C-ion irradiation at 10 Gy. Microarray analysis of irradiated lung tissue in three mouse strains identified differential expression changes of the growth differentiation factor 15 gene (Gdf15), that regulates inflammatory and apoptotic pathways and macrophage function and for the hyaluronan synthase 1 gene (Has1), that plays a role in hyaluronan metabolism. Immunohistochemical analysis showed that the ratio of CD44-positive cells as surrogate marker for hyaluronan accumulation and Mac3-positive cells as maker for inflammation and macrophage infiltration in irradiated lung varied significantly among three mouse strains. This study demonstrated the strain-dependent differential response in mice to high-LET C-ion irradiation. Our findings identified possible molecules relating to the strain-variance of lung toxicity after C-ion irradiation. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research(ICRR’2011) | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-01 | |||||
| 日付タイプ | Issued | |||||
