{"_buckets": {"deposit": "6184cbb9-8038-48b0-a2ed-6b65ceb0de0c"}, "_deposit": {"created_by": 1, "id": "70464", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "70464"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00070464", "sets": ["28"]}, "author_link": ["691973", "691976", "691975", "691977", "691974", "691978"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2011-06-17", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Hydroxyl radical (\u0026#8226;OH) is the most reactive species of reactive oxygen species (ROS); therefore, \u0026#8226;OH is one of the important player in oxidative stress. The \u0026#8226;OH can be measured using electron paramagnetic resonance (EPR) spin-trapping method. Generally, \u0026#8226;OH-scavenging-effect of an anti-oxidant has been estimated by a method adding the anti-oxidant and a spin-trapping agent to a \u0026#8226;OH generation system. Fenton reaction system or irradiating UV to hydrogen peroxide (H2O2) has been often used as the \u0026#8226;OH generation system. Using Fenton reaction system for estimating \u0026#8226;OH-scavenging-effect, iron-chelating effect of the anti-oxidant and/or reaction of H2O2 with the anti-oxidant must be considered to estimate the \u0026#8226;OH-scavenging-effect correctly. For using UV+H2O2 reaction system, reaction of H2O2 with the anti-oxidant and absorption of UV by the anti-oxidant must be considered for correct estimation of the \u0026#8226;OH-scavenging-effect. X-ray irradiation to water can also generate \u0026#8226;OH. In this simple system, estimation of the \u0026#8226;OH-scavenging-effect of an anti-oxidant can be much easier, while reduction of spin adduct with the anti-oxidant must be considered. In this study, we are trying to propose an efficient method for estimating \u0026#8226;OH-scavenging-effect of an anti-oxidant.\nA reaction mixture containing 30 mM of a spin-trapping agent (DMPO) and an arbitrary concentration of an anti-oxidant was prepared using 100 mM phosphate buffer (pH7, containing 0.05 mM DTPA). X-ray was irradiated to the reaction mixture and then amount of the \u0026#8226;OH-spin-adduct (DMPO-OH) was measured immediately using X-band EPR spectrometer. A reaction mixture containing 60 mM was prepared and irradiated by X-ray, then the anti-oxidant was added to estimate reduction of DMPO-OH by the anti-oxidant.\nX-ray irradiation to the reaction mixture containing DMPO generates DMPO-OH in the reaction mixture dose dependently. Even doing the experiment under N2 gas atmosphere, no difference was obtained for amount of DMPO-OH generated compared to the experiment in the air. The DMPO-OH obtained in the reaction mixture was from \u0026#8226;OH generated by X-ray. When anti-oxidant 1 was added to the reaction mixture, amount of X-ray induced DMPO-OH in the reaction mixture was decreased depending on the dose of the anti-oxidant 1. To determine whether the anti-oxidant 1 was really scavenging \u0026#8226;OH, reduction of DMPO-OH caused by the anti-oxidant 1 was estimated. The anti-oxidant 1 was added to the reaction mixture containing DMPO-OH, which was previously generated by X-ray irradiation. The reduction rate of DMPO-OH by the anti-oxidant 1 was enough slow; therefore, the reducing DMPO-OH amount by adding anti-oxidant before X-ray irradiation was mainly due to scavenging \u0026#8226;OH by the anti-oxidant 1. Anti-oxidant 2 also showed the reducing DMPO-OH amount, when the anti-oxidant 2 was added to reaction mixture before X-ray irradiation. Anti-oxidant 2, however, showed a rapid reduction of DMPO-OH, when the anti-oxidant 2 was added after X-ray irradiation. Scavenging \u0026#8226;OH by anti-oxidant 2 can not estimated directly from the result of the experiment; however, it is possible to think that anti-oxidant 2 was a strong reductant which can be reduce relatively stable nitroxyl radical (DMPO-OH in this case). Using the method in this experiment, anti-oxidative property can be estimated using scavenging \u0026#8226;OH and/or reducing DMPO-OH.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要（会議名, 開催地, 会期, 主催者等）", "attribute_value_mlt": [{"subitem_description": "4th International Symposium on Nutrition, Oxygen Biology and Medicine", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsumoto, Kenichiro"}], "nameIdentifiers": [{"nameIdentifier": "691973", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "691974", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ozawa, Toshihiko"}], "nameIdentifiers": [{"nameIdentifier": "691975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本 謙一郎", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "691976", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "中西 郁夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "691977", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小澤 俊彦", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "691978", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Method for Assessig Hydroxyl Radical Scavenging Activity", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Method for Assessig Hydroxyl Radical Scavenging Activity"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/70464", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-06-28"}, "publish_date": "2011-06-28", "publish_status": "0", "recid": "70464", "relation": {}, "relation_version_is_last": true, "title": ["Method for Assessig Hydroxyl Radical Scavenging Activity"], "weko_shared_id": -1}