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Method for Assessig Hydroxyl Radical Scavenging Activity

https://repo.qst.go.jp/records/70464
https://repo.qst.go.jp/records/70464
f9c92b6d-da8c-4e06-9c48-8d96f66f2a12
Item type 会議発表用資料 / Presentation(1)
公開日 2011-06-28
タイトル
タイトル Method for Assessig Hydroxyl Radical Scavenging Activity
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Matsumoto, Kenichiro

× Matsumoto, Kenichiro

WEKO 691973

Matsumoto, Kenichiro

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Nakanishi, Ikuo

× Nakanishi, Ikuo

WEKO 691974

Nakanishi, Ikuo

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Ozawa, Toshihiko

× Ozawa, Toshihiko

WEKO 691975

Ozawa, Toshihiko

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松本 謙一郎

× 松本 謙一郎

WEKO 691976

en 松本 謙一郎

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中西 郁夫

× 中西 郁夫

WEKO 691977

en 中西 郁夫

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小澤 俊彦

× 小澤 俊彦

WEKO 691978

en 小澤 俊彦

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抄録
内容記述タイプ Abstract
内容記述 Hydroxyl radical (•OH) is the most reactive species of reactive oxygen species (ROS); therefore, •OH is one of the important player in oxidative stress. The •OH can be measured using electron paramagnetic resonance (EPR) spin-trapping method. Generally, •OH-scavenging-effect of an anti-oxidant has been estimated by a method adding the anti-oxidant and a spin-trapping agent to a •OH generation system. Fenton reaction system or irradiating UV to hydrogen peroxide (H2O2) has been often used as the •OH generation system. Using Fenton reaction system for estimating •OH-scavenging-effect, iron-chelating effect of the anti-oxidant and/or reaction of H2O2 with the anti-oxidant must be considered to estimate the •OH-scavenging-effect correctly. For using UV+H2O2 reaction system, reaction of H2O2 with the anti-oxidant and absorption of UV by the anti-oxidant must be considered for correct estimation of the •OH-scavenging-effect. X-ray irradiation to water can also generate •OH. In this simple system, estimation of the •OH-scavenging-effect of an anti-oxidant can be much easier, while reduction of spin adduct with the anti-oxidant must be considered. In this study, we are trying to propose an efficient method for estimating •OH-scavenging-effect of an anti-oxidant.
A reaction mixture containing 30 mM of a spin-trapping agent (DMPO) and an arbitrary concentration of an anti-oxidant was prepared using 100 mM phosphate buffer (pH7, containing 0.05 mM DTPA). X-ray was irradiated to the reaction mixture and then amount of the •OH-spin-adduct (DMPO-OH) was measured immediately using X-band EPR spectrometer. A reaction mixture containing 60 mM was prepared and irradiated by X-ray, then the anti-oxidant was added to estimate reduction of DMPO-OH by the anti-oxidant.
X-ray irradiation to the reaction mixture containing DMPO generates DMPO-OH in the reaction mixture dose dependently. Even doing the experiment under N2 gas atmosphere, no difference was obtained for amount of DMPO-OH generated compared to the experiment in the air. The DMPO-OH obtained in the reaction mixture was from •OH generated by X-ray. When anti-oxidant 1 was added to the reaction mixture, amount of X-ray induced DMPO-OH in the reaction mixture was decreased depending on the dose of the anti-oxidant 1. To determine whether the anti-oxidant 1 was really scavenging •OH, reduction of DMPO-OH caused by the anti-oxidant 1 was estimated. The anti-oxidant 1 was added to the reaction mixture containing DMPO-OH, which was previously generated by X-ray irradiation. The reduction rate of DMPO-OH by the anti-oxidant 1 was enough slow; therefore, the reducing DMPO-OH amount by adding anti-oxidant before X-ray irradiation was mainly due to scavenging •OH by the anti-oxidant 1. Anti-oxidant 2 also showed the reducing DMPO-OH amount, when the anti-oxidant 2 was added to reaction mixture before X-ray irradiation. Anti-oxidant 2, however, showed a rapid reduction of DMPO-OH, when the anti-oxidant 2 was added after X-ray irradiation. Scavenging •OH by anti-oxidant 2 can not estimated directly from the result of the experiment; however, it is possible to think that anti-oxidant 2 was a strong reductant which can be reduce relatively stable nitroxyl radical (DMPO-OH in this case). Using the method in this experiment, anti-oxidative property can be estimated using scavenging •OH and/or reducing DMPO-OH.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 4th International Symposium on Nutrition, Oxygen Biology and Medicine
発表年月日
日付 2011-06-17
日付タイプ Issued
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