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Reaction of nitroxyl radicals and GSH; pH deendence
https://repo.qst.go.jp/records/70334
https://repo.qst.go.jp/records/70334ea4798d4-6049-4a4f-a9d7-c68fdc8fca07
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-11-24 | |||||
| タイトル | ||||||
| タイトル | Reaction of nitroxyl radicals and GSH; pH deendence | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Kenichiro
× Matsumoto, Kenichiro× Nakanishi, Ikuo× Krishna, Murali× 松本 謙一郎× 中西 郁夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nitroxyl radicals were utilized as redox probe in a field of in vivo EPR spectroscopy and imaging. Recently, utility of nitroxyl radicals as redox sensitive contrast agents for functional MRI were proposed and were getting interests. Detail reaction of nitroxyl radicals with each biological molecule, such as glutathione (GSH), were however not enough investigated. We have investigated temperature dependent reaction of nitroxyl radical with coexisting GSH. GSH can react with 2 molecules of a nitroxyl radical, and can eliminate EPR signal completely. Addition of K3Fe(CN)6 to the reaction mixture can not recover EPR signal of nitroxyl radical after the reaction with GSH. In this study, pH dependence of reduction of several nitroxyl radicals (TEMPO, TEMPOL, TEMPONE, carboxy-PROXYL, and carbamoyl-PROXYL) with coexisting glutathione at 37[deg]C was tested. The decay profiles of nitroxyl radicals were depending on pH. The reduction of nitroxyl radicals become faster in pH6 compared with pH7, except carboxy-PROXYL was not react in lower pH. Almost no reduction of nitroxyl radicals occurred in pH8. However, mechanism of reaction of nitroxyl radical and GSH is still in progress. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SFRBM's 17th Annual Meeting/SFRRI's XV Biennial Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2010-11-21 | |||||
| 日付タイプ | Issued | |||||
