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Identification of mouse mutant cells exhibiting the plastic mutant phenotype
https://repo.qst.go.jp/records/70272
https://repo.qst.go.jp/records/702727cdd1a3d-1949-4ef9-8119-5857e524df8c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-10-07 | |||||
| タイトル | ||||||
| タイトル | Identification of mouse mutant cells exhibiting the plastic mutant phenotype | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Masatake
× Yamauchi, Masatake× Fukutsu, Kumiko× Sakagami, Mari× Yamada, Yuji× 山内 正剛× 福津 久美子× 坂上 万里× 山田 裕司 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate the initial processes involved in radiation carcinogenesis, we isolated and examined mouse mutant cells exhibiting phenotype plasticity. Approximately 10% of 6-thioguanine resistant (6TGR) cells derived from the irradiated cell population exhibited phenotype plasticity and reverted to wild type HAT resistance (HATR). Similar mutant cells were also identified in an un-irradiated wild type cell population, but at a lower frequency. Ionizing irradiation enhanced the frequency of the plastic mutation approximately 24 times in our experiment. Treatment with 5-aza-cytidine did not affect phenotype plasticity. Detailed molecular analysis of the promoter region of the hypoxanthine phosphoribosyl transferase (Hprt) gene revealed that most cytidine residues were not methylated, even in 6TGR mutant cells, in which Hprt activity must be down-regulated. These results suggested that DNA methylation was not involved in mutant phenotype plasticity. We have identified a new type of genomic instability induced by ionizing radiation. Plasticity in gene regulation may play an important role in radiation carcinogenesis, which is a multiple-stages process. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 欧州エピジェネティクス会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-15 | |||||
| 日付タイプ | Issued | |||||
