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Possible involvement of glucose metabolism regulation in insulin-mediated modulation of radiosensitivity in human breast cancer cells.
https://repo.qst.go.jp/records/70252
https://repo.qst.go.jp/records/70252f7d085b1-0a0f-49f7-9380-9d0ffd8ebdbc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-24 | |||||
| タイトル | ||||||
| タイトル | Possible involvement of glucose metabolism regulation in insulin-mediated modulation of radiosensitivity in human breast cancer cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Tetsuo
× Nakajima, Tetsuo× Nenoi, Mitsuru× 中島 徹夫× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Effects of low-dose radiation have been evaluated on the molecular levels, however, relationship of its change with biological phenomenon such as adaptive responses, radioresistance or carcinogenesis remains unclear. We have demonstrated that, in the presence of insulin, MCF7 cells (human breast cancer cells) appear to be sensitive to low-dose irradiation (0.25 Gy) and that it is likely due to growth inhibition after low-dose irradiation in the cells with insulin. As phosphatidylinositol 3 (PI3)-kinase is a known participant in insulin-related signaling cascades, effects of a PI3-kinase inhibitor on the insulin mediation were evaluated. LY294002, one of PI3-kinase inhibitors sensitized the cells to irradiation with 0.25 Gy in the absence of insulin, and its effect was similar to that mediated by insulin. In addition, microarray analyses in the presence or the absence of insulin were performed and three genes were identified as altered genes whose expressions were increased by more than two folds after irradiation in the absence of insulin but not in the presence of insulin. One of the altered genes is MEK2 (Mitogen-activated protein kinase kinase 2) and the others are glucose metabolism regulatory enzymes. However, PD98059, a MEK inhibitor, did not sensitize the cells in the absence of insulin. Roles of PI3 kinase or the altered gene expression in low-dose radiation-induced signaling cascades will be discussed. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 48th Annual Meeting of the Biophysical Society of Japan（第４８回日本生物物理学会年会） | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-22 | |||||
| 日付タイプ | Issued | |||||
